In this study, Ag nanoparticles were firstly reduced on the surface of lignin nanoparticles (LNP_Ag) by direct reaction of silver nitrate without the use of a catalyst. Thermogravimetric analysis, Zeta potential and transmission electron microscopy measurements were performed to give evidence of the effectiveness of the reaction. After that, glutaraldehyde crosslinked PVA hydrogels, were produced by addition of unmodified LNP and Ag loaded LNP (LNP_Ag) in presence of cellulose nanocrystals (CNC). Thermal, mechanical, rheological, microstructural and biological anti-oxidative and anti-bacterial properties of the resulted hydrogels were investigated. It was proved that all the three nanofillers were homogeneously dispersed in PVA, and the pore diameter of the hydrogels was in the range of 0.5–2.0 μm. Nevertheless, the hydrogels showed high toughness, long-term and repeatable adhesiveness to a variety of substrates. In particular, composite hydrogels containing LNP_Ag nanoparticles showed excellent radical scavenging and antibacterial activities. Consequently, the effects of PVA-2CNC-2LNP_Ag on wound healing in mice model of full-thickness skin resection were evaluated by hematoxylin and eosin staining, taking as a reference the PVA-2CNC-2LNP system. The results showed that the wound healing time of PVA-2CNC-2LNP_Ag group was faster than that of neat PVA and PVA-2CNC, highlighting the role of LNP_Ag in enhancing the contact-active anti-oxidative and antibacterial activities mechanism in composite hydrogels. We expected that PVA hydrogels incorporating LNP_Ag could be used as green and efficient biomedical wound dressing materials.

在这项研究中，Ag 纳米粒子首先通过硝酸银的直接反应在木质素纳米粒子 (LNP _Ag )的表面上进行还原，而不使用催化剂。进行热重分析、Zeta 电位和透射电子显微镜测量以提供反应有效性的证据。之后，戊二醛交联的 PVA 水凝胶通过添加未改性的 LNP 和负载 Ag 的 LNP（LNP _Ag) 在纤维素纳米晶体 (CNC) 的存在下。研究了所得水凝胶的热、机械、流变、微观结构和生物抗氧化和抗菌性能。结果表明，三种纳米填料均均匀地分散在 PVA 中，水凝胶的孔径在 0.5-2.0 μm 范围内。尽管如此，水凝胶仍显示出对各种基材的高韧性、长期和可重复的粘附性。特别是，含有 LNP _Ag纳米颗粒的复合水凝胶显示出优异的自由基清除和抗菌活性。因此，PVA-2CNC-2LNP _{Ag 的影响}以PVA-2CNC-2LNP系统为参考，通过苏木精和伊红染色评估全层皮肤切除小鼠模型伤口愈合的效果。结果表明，PVA-2CNC-2LNP _Ag组的伤口愈合时间比纯PVA和PVA-2CNC快，突出了LNP _Ag在增强复合水凝胶接触活性抗氧化和抗菌活性机制中的作用. 我们预计含有 LNP _{Ag 的}PVA 水凝胶可用作绿色高效的生物医学伤口敷料材料。