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Fabricating biopolymer-inorganic hybrid microspheres for enzyme immobilization: Connect membrane emulsification with biomimetic mineralization
Particuology ( IF 4.1 ) Pub Date : 2021-08-21 , DOI: 10.1016/j.partic.2021.07.006
Jian Li 1 , Tiantian Feng 1 , Lin Han 1 , Min Zhang 1 , Tao Jiang 1
Affiliation  

The organic-inorganic hybrid composites displayed great potential for biotechnological and biomedical application. In this research, a gelatin/alginate/silica hybrid microsphere was developed by a synergy of membrane emulsification process and biomimetic mineralization method. The gelatin was mixed and complexed with alginate solutions (water phase). The water phase was extruded through a Shirasu Porous Glass (SPG) membrane, and then was crosslinked, which formed gelatin/alginate microspheres. The biomimetic mineralization was occurred in situ by immersing gelatin/alginate complex in a Na2SiO3 solution, while silica was formed around the organic microspheres, resulting in the final gelatin/alginate/silica hybrid microspheres. These microspheres were characterized by SEM, TEM, EDS, TGA/DTA, and DSC. The hybrid microcapsules present a more than 40% mass fraction of the inorganic component, and displayed superior swelling resistance to biopolymer complex microspheres. Glycerol dehydrogenase (GlyDH) was immobilized in the obtained novel gelatin/alginate-silica hybrid microspheres as the model enzyme. Due to the protective effect of carriers, the pH tolerance stability, storage and recycling stability of the immobilized GlyDH were all improved in comparison with free GlyDH.



中文翻译:

制备用于酶固定化的生物聚合物-无机杂化微球:将膜乳化与仿生矿化连接起来

有机-无机杂化复合材料在生物技术和生物医学应用方面显示出巨大的潜力。在这项研究中,通过膜乳化工艺和仿生矿化方法的协同作用开发了一种明胶/海藻酸盐/二氧化硅杂化微球。将明胶与藻酸盐溶液(水相)混合并复合。水相通过 Shirasu 多孔玻璃 (SPG) 膜挤出,然后交联,形成明胶/藻酸盐微球。通过将明胶/藻酸盐复合物浸入 Na 2 SiO 3 中,原位发生仿生矿化溶液,而在有机微球周围形成二氧化硅,从而形成最终的明胶/藻酸盐/二氧化硅混合微球。这些微球通过 SEM、TEM、EDS、TGA/DTA 和 DSC 进行表征。混合微胶囊的无机组分质量分数超过 40%,并且对生物聚合物复合微球显示出优异的抗溶胀性。将甘油脱氢酶(GlyDH)固定在获得的新型明胶/藻酸盐-二氧化硅杂化微球中作为模型酶。由于载体的保护作用,与游离GlyDH相比,固定化GlyDH的pH耐受稳定性、储存和循环稳定性均有所提高。

更新日期:2021-08-21
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