Microchimica Acta ( IF 5.3 ) Pub Date : 2021-08-20 , DOI: 10.1007/s00604-021-04946-3 O Amor-Gutiérrez 1 , E Costa-Rama 1 , M T Fernández-Abedul 1
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An electroanalytical platform capable to take and dilute the sample has been designed in order to fully integrate the different steps of the analytical process in only one device. The concept is based on the addition of glass-fiber pads for sampling and diluting to an electrochemical cell combining a paper-based working electrode with low-cost connector headers as counter and reference electrodes. In order to demonstrate the feasibility of this all-in-one platform for biosensing applications, an enzymatic sensor for glucose determination (requiring a potential as low as −0.1 V vs. gold-plated wire by using ferrocyanide as mediator) was developed. Real food samples, such as cola beverages and orange juice, have been analyzed with the bioelectroanalytical lab-on-paper platform. As a proof-of-concept, and trying to go further in the integration of steps, sucrose was successfully detected by depositing invertase in the sampling strip. This enzyme hydrolyzes sucrose into fructose and glucose, which was determined using the enzymatic biosensor. This approach opens the pathway for the development of devices applying the lab-on-paper concept, saving costs and time, and making possible to perform decentralized analysis with high accuracy.
Graphical abstract
中文翻译：
用于葡萄糖传感的电化学纸基设备中完全集成的采样器和稀释器
设计了一种能够采集和稀释样品的电分析平台，以便将分析过程的不同步骤完全集成到一个设备中。该概念基于在电化学电池中添加用于采样和稀释的玻璃纤维垫，将纸基工作电极与低成本连接器接头作为反电极和参考电极相结合。为了证明这种多合一平台在生物传感应用中的可行性，开发了一种用于葡萄糖测定的酶传感器（通过使用亚铁氰化物作为介质，相对于镀金线需要低至 -0.1 V 的电位）。可乐饮料和橙汁等真实食品样品已使用生物电分析纸上实验室平台进行了分析。作为概念验证，并试图在步骤的整合上走得更远，通过将蔗糖沉积在采样条中成功检测到蔗糖。这种酶将蔗糖水解成果糖和葡萄糖，这是使用酶生物传感器测定的。这种方法为应用纸上实验室概念的设备开发开辟了道路，节省了成本和时间，并可以进行高精度的分散分析。
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