Multiple sclerosis (MS) is a neurodegenerative disease with various factors affecting its etiology. Overproduction of nitric oxide and subsequent lesions of biopolymers are some of the possible causes of the disease. This study aimed to measure the most relevant nitrosative and oxidative stress biomarkers and the level of modified DNA bases in patients with MS. Each parameter was assayed in 25 patients with MS and 25 healthy controls. This study involved detecting blood plasma and serum nitric oxide metabolites by chemiluminescence detector Sievers NOA-280i, malondialdehyde (MDA) measurements with thiobarbituric acid reactive substance (TBARS) assay, detection of oxidized purines and pyrimidines with the enzyme-modified comet assay. Statistical analysis of the results was performed by one-way analysis of variance (ANOVA) and unpaired t test for the comparison of less than three data sets. DNA single-strand breaks, levels of modified purines and pyrimidines, as well as nitrite and nitrate levels in plasma and serum samples, were significantly higher in patients with MS than in healthy controls. On the contrary, MDA levels appeared to be lower in patients with MS.

多发性硬化症 (MS) 是一种神经退行性疾病，其病因受到多种因素的影响。一氧化氮的过量产生和随后的生物聚合物损伤是该疾病的一些可能原因。本研究旨在测量 MS 患者中最相关的亚硝化和氧化应激生物标志物以及修饰的 DNA 碱基水平。在 25 名 MS 患者和 25 名健康对照中测定了每个参数。本研究涉及通过化学发光检测器 Sievers NOA-280i 检测血浆和血清一氧化氮代谢物，采用硫代巴比妥酸反应物质 (TBARS) 测定法测定丙二醛 (MDA)，采用酶修饰彗星测定法检测氧化的嘌呤和嘧啶。通过单向方差分析 (ANOVA) 和非配对 t 检验对少于三个数据集的比较进行结果的统计分析。MS 患者的 DNA 单链断裂、修饰的嘌呤和嘧啶水平以及血浆和血清样本中的亚硝酸盐和硝酸盐水平显着高于健康对照组。相反，MS 患者的 MDA 水平似乎较低。