ABSTRACT

Ionizing radiation hydrolyzes H₂O and leads to free radicals production that initiates inflammation and DNA damages. Siwa dates are an important crop in Egypt that has been used as a staple food due to its nutritional value and its antioxidant properties. The study aimed to investigate the radioprotective effects of aqueous extract of Siwa dates in whole body γ- irradiated mice. Animals were divided into four groups: healthy mice, unirradiated mice that received Siwa date aqueous extract, irradiated unprotected mice and irradiated mice that received daily 4 ml/kg Siwa date extract for 14 days prior to irradiation. Twenty four hours post irradiation, blood and liver samples were collected for immunological, biochemical, and histopathological studies. Apoptosis and apoptotic proteins in hepatocytes were measured by flow cytometry techniques. Moreover, the cytokinesis-blocked micronucleus assay was performed to measure DNA damages. Matrix metalloproteinases (MMPs) were evaluated in liver samples by both ELISA and immunohistochemical techniques. The results have shown the improvement in liver histopathological sections and the amelioration in the pro-inflammatory cascade. Siwa date extract allowed DNA protection from the destructive effect of irradiation. In conclusion, Siwa dates were effective in alleviating radiation induced damages such as hepatotoxicity, oxidative stress, inflammation, and DNA damage.

摘要

电离辐射水解 H ₂O 并导致自由基产生，引发炎症和 DNA 损伤。西瓦枣是埃及的一种重要作物，因其营养价值和抗氧化特性而被用作主食。该研究旨在研究西瓦枣水提取物对全身 γ 辐射小鼠的辐射防护作用。动物被分为四组：健康小鼠、接受西瓦枣水提取物的未辐照小鼠、未受保护的辐照小鼠和在辐照前每天接受 4 ml/kg 西瓦枣提取物 14 天的辐照小鼠。照射后 24 小时，收集血液和肝脏样本用于免疫学、生化和组织病理学研究。通过流式细胞术技术测量肝细胞中的凋亡和凋亡蛋白。此外，进行胞质分裂阻断微核试验以测量 DNA 损伤。通过 ELISA 和免疫组织化学技术评估肝脏样品中的基质金属蛋白酶 (MMP)。结果表明肝脏组织病理切片的改善和促炎级联反应的改善。Siwa 日期提取物可以保护 DNA 免受辐射的破坏性影响。总之，西瓦枣可有效减轻辐射引起的损伤，如肝毒性、氧化应激、炎症和 DNA 损伤。Siwa 日期提取物可以保护 DNA 免受辐射的破坏性影响。总之，西瓦枣可有效减轻辐射引起的损伤，如肝毒性、氧化应激、炎症和 DNA 损伤。Siwa 日期提取物可以保护 DNA 免受辐射的破坏性影响。总之，西瓦枣可有效减轻辐射引起的损伤，如肝毒性、氧化应激、炎症和 DNA 损伤。