Ovarian cancer, a severe lethal gynecological malignancy, is characterized by both high morbidity and mortality. Long noncoding RNAs (lncRNAs) have recently caused extensive concern due to their regulatory function in various human tumors. There are a mounting number of lncRNAs that are in extreme need of research, serving as biomarkers for diagnosis and therapy for ovarian cancer. In the present study, RT‑qPCR was employed to detect how Rhophilin Rho GTPase binding protein 1 antisense RNA1 (RHPN1‑AS1), miR‑6884‑5p and DNA topoisomerase IIα (TOP2A) are expressed in ovarian cancer tissues or cell lines. BrdU, MTT, colony formation and cell adhesion assays, caspase‑3 activity, flow cytometry and wound healing assay were employed to assess cell proliferation, viability, colony number, adhesion, apoptosis and migration in ovarian cancer, respectively. RHPN1‑AS1 was determined to be enriched in ovarian cancer tissues and cell lines. Silencing of RHPN1‑AS1 was reported to increase cell apoptosis and impair cell proliferation, viability, colony number, adhesion and migration in vitro. Furthermore, RHPN1‑AS1 was able to sponge miR‑6884‑5p which directly targets TOP2A in ovarian cancer. Notably, silencing of RHPN1‑AS1 functionally reversed the oncogenic effect induced by the miR‑6884‑5p inhibitor, while the miR‑6884‑5p inhibitor markedly restored the inhibition of ovarian carcinogenesis modulated by silencing TOP2A in ovarian cancer. RHPN1‑AS1 was found to promote ovarian carcinogenesis via sponging miR‑6884‑5p thus releasing TOP2A, and RHPN1‑AS1 may act as a promising biomarker for the prognosis and therapy of ovarian cancer.

中文翻译：

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RHPN1-AS1 通过海绵 miR-6884-5p 从而释放 TOP2A mRNA 来促进卵巢癌发生。

卵巢癌是一种严重的致死性妇科恶性肿瘤，其特点是发病率和死亡率都很高。长链非编码 RNA (lncRNAs) 最近因其在各种人类肿瘤中的调节功能而引起广泛关注。越来越多的 lncRNA 非常需要研究，可作为诊断和治疗卵巢癌的生物标志物。在本研究中，RT-qPCR 用于检测 Rhophilin Rho GTPase 结合蛋白 1 反义 RNA1 (RHPN1-AS1)、miR-6884-5p 和 DNA 拓扑异构酶 IIα (TOP2A) 在卵巢癌组织或细胞系中的表达情况。BrdU、MTT、集落形成和细胞粘附试验、caspase-3活性、流式细胞术和伤口愈合试验分别用于评估卵巢癌中的细胞增殖、活力、集落数、粘附、凋亡和迁移。RHPN1-AS1 被确定在卵巢癌组织和细胞系中富集。据报道，RHPN1-AS1 的沉默会增加细胞凋亡并损害细胞增殖、活力、集落数、粘附和迁移体外。此外，RHPN1-AS1 能够吸收直接靶向卵巢癌 TOP2A 的 miR-6884-5p。值得注意的是，RHPN1-AS1 的沉默在功能上逆转了 miR-6884-5p 抑制剂诱导的致癌作用，而 miR-6884-5p 抑制剂显着恢复了通过沉默 TOP2A 在卵巢癌中调节的卵巢癌发生的抑制作用。RHPN1-AS1 被发现通过海绵 miR-6884-5p 促进卵巢癌发生，从而释放 TOP2A，并且 RHPN1-AS1 可能作为卵巢癌预后和治疗的有希望的生物标志物。