Diabetic foot ulcer (DFU) is biggest life threats globally and increases their severity increases health complications for health of patients. The present study was investigated to recover the wound healing activity of baicalin in STZ-induced DFU rats by evaluating biochemical and molecular markers. The experimental animals induced with diabetes and excision wounds were treated with different doses of baicalin (25, 50, and 100 mg/kg). The serum glucose level, body weight and food intake were measured. In addition, DFU rat groups showed decreased food intake and increased body weight. The tissue was subjected to biochemical evaluation, histopathology, quantitative polymerase chain reaction and Western blot analysis. Histopathology reports revealed that diabetic wound control (DWC) + baicalin (100 mg/kg) treated group showed more than 90% recovery with more epithelization and remarkably improved angiogenesis and infiltration of the inflammatory cells. In this study we also proved that upregulated the p-ERK, ERK, HSP27, and p-HSP27 protein expression and mRNA expression of Ang-1, VEGF-c, TGF-β, Tie-2, and SMAD2/3 implicating the potential antidiabetic and wound healing property of baicalin. Thus, baicalin is a potential therapeutic candidate for a diabetic foot ulcer and chronic wounds treatment.

中文翻译：

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黄芩苷调节 VEGF-c、Ang-1/Tie2、TGF-β 和 Smad2/3 mRNA 表达抑制链脲佐菌素诱导的糖尿病足溃疡大鼠创面愈合

糖尿病足溃疡 (DFU) 是全球最大的生命威胁，其严重程度的增加会增加患者健康的并发症。本研究通过评估生化和分子标志物来研究黄芩苷在 STZ 诱导的 DFU 大鼠中的伤口愈合活性。用不同剂量的黄芩苷（25、50和100 mg/kg）治疗糖尿病和切除伤口诱导的实验动物。测量血清葡萄糖水平、体重和食物摄入量。此外，DFU 大鼠组表现出食物摄入减少和体重增加。对组织进行生化评估、组织病理学、定量聚合酶链反应和蛋白质印迹分析。组织病理学报告显示，糖尿病伤口控制 (DWC) + 黄芩苷 (100 mg/kg) 治疗组显示超过 90% 的恢复，更多的上皮化和显着改善的血管生成和炎症细胞的浸润。在本研究中我们还证明了上调 p-ERK、ERK、HSP27 和 p-HSP27 蛋白表达和 Ang-1、VEGF-c、TGF-β、Tie-2 和 SMAD2/3 表明黄芩苷具有潜在的抗糖尿病和伤口愈合特性。因此，黄芩苷是糖尿病足溃疡和慢性伤口治疗的潜在治疗候选者。