Epidural fibrosis after lumbar laminectomy refers to a serious complication, and excessive proliferation of fibroblasts is considered the major factor. Interferon-alpha-2b (IFN-α-2b) can exert antiviral and antiproliferative effects, which has been suggested to effectively prevent several fibrotic diseases. However, the effect of IFN-α-2b on the prevention of epidural fibrosis (EF) and its possible mechanism remain unclear. In this study, in vitro and in vivo experiments were performed to examine the possible mechanism of IFN-α-2b for preventing EF. Cell counting kit-8 (CCK-8), cell cycle test, Edu incorporation, wound healing assay, transwell test, and Western blotting assay were performed to investigate the inhibitory effect of IFN-α-2b on the proliferation and migration of fibroblasts in vitro. As indicated from the results, IFN-α-2b was capable of inhibiting proliferation and migration of fibroblasts and inhibiting the activity of the transforming growth factor β (TGFβ)/Smad signaling pathway. In vivo, the effect of IFN-α-2b on the reduction of EF was determined by performing histological macroscopic evaluation and histological and immunohistochemical staining. As suggested from the results, IFN-α-2b significantly inhibited EF after laminectomy. As revealed from the mentioned results, IFN-α-2b may have a promising application for preventing EF in the future.

中文翻译：

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IFN-α-2b 通过 TGFβ/Smad 信号通路抑制成纤维细胞的增殖和迁移以减少术后硬膜外纤维化

腰椎板切除术后硬膜外纤维化是一种严重的并发症，其中成纤维细胞过度增殖被认为是主要因素。干扰素-α-2b (IFN-α-2b) 可发挥抗病毒和抗增殖作用，已被认为可有效预防多种纤维化疾病。然而，IFN-α-2b对预防硬膜外纤维化（EF）的作用及其可能的机制仍不清楚。在这项研究中，体外和体内进行了实验以检查 IFN-α-2b 预防 EF 的可能机制。采用细胞计数试剂盒8（CCK-8）、细胞周期试验、Edu掺入、伤口愈合试验、transwell试验和Western blotting试验研究IFN-α-2b对成纤维细胞增殖和迁移的抑制作用。体外。结果表明，IFN-α-2b能够抑制成纤维细胞的增殖和迁移，抑制转化生长因子β（TGFβ）/Smad信号通路的活性。体内，通过进行组织学宏观评估和组织学和免疫组织化学染色来确定 IFN-α-2b 对 EF 减少的影响。结果表明，IFN-α-2b 在椎板切除术后显着抑制 EF。正如上述结果所揭示的，IFN-α-2b 可能在未来预防 EF 方面具有广阔的应用前景。