Background

House dust mite (HDM) allergens are major elicitors of allergic reactions worldwide.

Objective

Identification, characterization, and evaluation of diagnostic utility of a new important HDM allergen was performed.

Methods

A cDNA coding for a new Dermatophagoides pteronyssinus (Dp) allergen, Der p 37, was isolated from a Dp expression library with allergic patients’ IgE antibodies. Recombinant Der p 37 (rDer p 37) expressed in Escherichia coli was purified, then characterized by mass spectrometry, circular dichroism, and IgE reactivity by ImmunoCAP ISAC technology with sera from 111 clinically defined HDM-allergic patients. The allergenic activity of rDer p 37 was studied by basophil activation and CD4⁺ T-cell responses by carboxyfluorescein diacetate succinimidyl ester dilution assays. Specific antibodies raised against rDer p 37 were used for the ultrastructural localization of Der p 37 in mites by immunogold transmission electron microscopy.

Results

Der p 37, a 26 kDa allergen with homology to chitin-binding proteins, is immunologically distinct from Der p 15, 18, and 23. It is located in the peritrophic membrane of fecal pellets. Der p 37 reacted with IgE antibodies from a third of HDM-allergic patients and induced specific basophil- and CD4⁺ T-cell activation. Der p 37 IgE–positive patients had significantly higher IgE levels to major HDM allergens, reacted with more HDM allergens, and had a higher risk (odds ratio = 3.1) of asthma compared to Der p 37–negative patients.

Conclusions

Der p 37, a new Dp allergen recognized by a third of HDM-allergic patients, may serve as a surrogate marker for severe HDM sensitization and asthma.

中文翻译：

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IgE 识别屋尘螨过敏原 Der p 37 与哮喘有关

背景

屋尘螨 (HDM) 过敏原是全球过敏反应的主要诱因。

客观的

对一种新的重要 HDM 过敏原的诊断效用进行了鉴定、表征和评估。

方法

从具有过敏患者 IgE 抗体的 Dp 表达文库中分离出编码新的Dermatophagoides pteronyssinus (Dp) 过敏原 Der p 37 的 cDNA。纯化在大肠杆菌中表达的重组 Der p 37 (rDer p 37) ，然后用来自 111 名临床定义的 HDM 过敏患者的血清通过 ImmunoCAP ISAC 技术进行质谱、圆二色谱和 IgE 反应性的表征。rDer p 37 的致敏活性通过嗜碱性粒细胞活化和通过羧基荧光素二乙酸琥珀酰亚胺酯稀释测定的 CD4 ⁺ T 细胞反应进行了研究。针对 rDer p 37 产生的特异性抗体用于通过免疫金透射电子显微镜对螨虫中的 Der p 37 进行超微结构定位。

结果

Der p 37 是一种 26 kDa 的过敏原，与几丁质结合蛋白具有同源性，在免疫学上与 Der p 15、18 和 23 不同。它位于粪便颗粒的围营养膜中。Der p 37 与来自三分之一 HDM 过敏患者的 IgE 抗体反应，并诱导特异性嗜碱性粒细胞和 CD4 ⁺ T 细胞活化。与 Der p 37 阴性患者相比，Der p 37 IgE 阳性患者对主要 HDM 过敏原的 IgE 水平显着升高，与更多 HDM 过敏原发生反应，并且哮喘风险（优势比 = 3.1）更高。

结论

Der p 37 是一种新的 Dp 过敏原，被三分之一的 HDM 过敏患者识别，可作为严重 HDM 致敏和哮喘的替代标志物。