The study aimed to identify the male-fertility restoration locus (Ms) among 72 breeding lines of onion (Allium cepa L.) by genotyping, which is crucial in the development of F₁ hybrid onions using cytoplasmic-genic male sterility (CGMS) system. Thus, two markers were used to identify the Ms locus, the simple PCR marker namely jnurf20 is dominant nature cover across the breeding lines genetic backgrounds, whereas the PsaO gene-specific marker could not spread all along the breeding lines evaluated in the study. Since all reported markers are may or may not have marker genotype association across different genetic backgrounds of onion breeding lines. However, these molecular markers are highly useful in the marker-assisted selection of Ms locus. Thus, this study reveals the amplification of validated markers among the breeding lines used, the study essential to advance the marker-assisted breeding for the development of F₁ hybrid onions in near future.

该研究旨在通过基因分型确定72 个洋葱 ( Allium cepa L.)育种品系中的雄性生育力恢复位点 ( Ms ) ，这对于使用细胞质基因雄性不育 (CGMS) 系统开发 F ₁杂交洋葱至关重要。. 因此，使用两个标记来识别Ms基因座，简单的 PCR 标记即 jnurf20 是整个育种系遗传背景的显性自然覆盖，而PsaO基因特异性标记不能沿着研究中评估的育种系传播。由于所有报告的标记在洋葱育种系的不同遗传背景中可能具有也可能不具有标记基因型关联。然而，这些分子标记在Ms基因座的标记辅助选择中非常有用。因此，本研究揭示了所使用育种品系中已验证标记的扩增，该研究对于在不久的将来推进标记辅助育种以开发 F ₁杂交洋葱至关重要。