Abstract

Non-enzymatic glucose biosensors have attracted considerable attention due to their approved benefits. The present study aims to design and produced a non-enzymatic biosensor based on polyacrylonitrile/polyaniline/CuO nanofibrous composites on indium-tin oxide (ITO) substrate using single-step electrospinning at different conditions. Electron microscopic studies show that continuous and uniform fibers from an optimum blend of PAN/PANI (90/10 mass ratio) incorporating with 5% CuO were successfully fabricated under 20 kV and at 18 cm. By employing of FTIR and XRD, characteristic peaks of chemical and crystalline structures of polymers and CuO were detected, which indicate successful incorporation of CuO into the nanofibers. Electrochemical studies, including cyclic voltammetry followed by amprometry have also been performed to evaluate the performance of the biosensor. The prepared electrode showed a linear response (R² = 0.9983) over a wide concentration range from 1 × 10⁻⁵ to 3 × 10⁻³M. The detection limit based on signal to noise ratio of 3 was 1.2 × 10⁻⁶M.

摘要

非酶葡萄糖生物传感器由于其公认的益处而引起了相当多的关注。本研究旨在设计和生产一种基于聚丙烯腈/聚苯胺/CuO纳米纤维复合材料在氧化铟锡（ITO）基底上的非酶生物传感器，采用单步静电纺丝在不同条件下进行。电子显微镜研究表明，在 20 kV 和 18 cm 下成功地制造了由 PAN/PANI（质量比为 90/10）与 5% CuO 的最佳混合物制成的连续且均匀的纤维。通过FTIR和XRD，检测到聚合物和CuO的化学结构和晶体结构的特征峰，表明CuO成功地掺入到纳米纤维中。电化学研究，包括循环伏安法和安培法也已用于评估生物传感器的性能。制备的电极显示出线性响应（R² = 0.9983) 在从 1 × 10 ^-5到 3 × 10 ^-3 M 的宽浓度范围内。基于信噪比 3 的检测限为 1.2 × 10 ^-6 M。