Tumors of the submandibular salivary gland (SMG) are uncommon but sufficiently frequent for the physician to consider them in routine examinations and for the pathologist to be prepared to differentiate them from other tissue abnormalities. However, scarcity of specimens makes training difficult, a situation compounded by the lack of accepted universal diagnostic guidelines. Furthermore, there is little information on the chaperone system (CS) of the gland, despite the increasing evidence of its participation in carcinogenesis as a biomarker for diagnosis and patient follow up, and in the mechanisms by which the tumor cells thrive. We are investigating this aspect of various tumors, and here we describe standardized methods for assessing the tissue levels of two chaperones, Hsp27 and Hsp60, in normal SMG and its tumors. We present illustrative results obtained with immunohistochemistry (IHC) and immunofluorescence-confocal microscopy (IF-CM), which we propose as a platform onto which a data base could be built by adding new information and which would provide material for developing guidelines for tumor identification and monitoring. The initial findings are encouraging in as much as the tumors surveyed showed quantitative patterns of Hsp27 and Hsp60 that distinguished tumoral from normal tissue and certain tumors from the others, and the results from IHC were confirmed by IF-CM.

下颌下唾液腺 (SMG) 肿瘤并不常见，但其频率足以让医生在常规检查中考虑它们，并让病理学家做好将它们与其他组织异常区分开来的准备。然而，标本稀缺使培训变得困难，缺乏公认的通用诊断指南使情况更加复杂。此外，关于腺体伴侣系统 (CS) 的信息很少，尽管越来越多的证据表明它作为诊断和患者随访的生物标志物参与致癌作用，以及肿瘤细胞生长的机制。我们正在研究各种肿瘤的这一方面，在这里我们描述了评估正常 SMG 及其肿瘤中两种伴侣 Hsp27 和 Hsp60 的组织水平的标准化方法。我们展示了通过免疫组织化学 (IHC) 和免疫荧光共聚焦显微镜 (IF-CM) 获得的说明性结果，我们建议将其作为一个平台，在该平台上可以通过添加新信息来建立数据库，并为制定肿瘤识别指南提供材料和监测。初步研究结果令人鼓舞，因为所调查的肿瘤显示出 Hsp27 和 Hsp60 的定量模式，可将肿瘤与正常组织以及某些肿瘤与其他组织区分开来，并且 IHC 的结果得到了 IF-CM 的证实。我们建议将其作为一个平台，在该平台上可以通过添加新信息来建立数据库，并为制定肿瘤识别和监测指南提供材料。初步研究结果令人鼓舞，因为所调查的肿瘤显示出 Hsp27 和 Hsp60 的定量模式，可将肿瘤与正常组织以及某些肿瘤与其他组织区分开来，并且 IHC 的结果得到了 IF-CM 的证实。我们建议将其作为一个平台，在该平台上可以通过添加新信息来建立数据库，并为制定肿瘤识别和监测指南提供材料。初步研究结果令人鼓舞，因为所调查的肿瘤显示出 Hsp27 和 Hsp60 的定量模式，可将肿瘤与正常组织以及某些肿瘤与其他组织区分开来，并且 IHC 的结果得到了 IF-CM 的证实。