H. boliviensis strain kknpp38 is a dense exopolysaccharide (EPS) producing bacterium, isolated from the early-stage (72-h-old) of marine biofilm. Laboratory experiments demonstrated that this isolate forms a potent biofilm on various artificial substrata viz. polystyrene, stainless steel as well as titanium and possesses high tolerance to chlorine disinfection. To determine the genes and biosynthetic pathways involved in the EPS production, whole-genome sequencing was performed using high-throughput Illumina tag sequencing. The high-quality reads were first de novo assembled using Unicycler genome assembler (version 0.4.9b) and then annotated using Prokka (version 1.13). The complete genome comes from one circular chromosome containing 4.96 Mbp DNA with G + C content of 55%, and encompasses genes encoding 4476 proteins, 2 rRNAs, and 57 tRNAs. Intriguingly, genomic analysis revealed the existence of genes involved in ATP-binding cassette (ABC) transporter-dependent EPS biosynthesis pathways (ugd, ugd2, galU). In addition, we identified genes involved in ectoine (ectA, ectB, ectC, ectD) and polyhydroxyalkanoates (PHAs; fabA, fabB, fabD, fabF, fabH, fabV, fabZ, phaC, phaD, phaG, phaR, phaZ1) production, which are known to involve in bacterial adaptation in saline environment. The outcomes of this study expand scientific understanding on the genes and pathways involved in EPS biosynthesis by marine bacteria.

H. boliviensis菌株 kknpp38 是一种致密的胞外多糖 (EPS) 生产细菌，从海洋生物膜的早期 (72-h) 中分离出来。实验室实验表明，这种分离物在各种人工基质上形成了有效的生物膜，即。聚苯乙烯、不锈钢和钛，对氯消毒具有很高的耐受性。为了确定 EPS 生产中涉及的基因和生物合成途径，使用高通量 Illumina 标签测序进行全基因组测序。高质量的读数首先是从头开始的使用 Unicycler 基因组组装器（版本 0.4.9b）组装，然后使用 Prokka（版本 1.13）进行注释。完整的基因组来自一个环状染色体，其中包含 4.96 Mbp DNA 和 55% 的 G + C 含量，包含编码 4476 个蛋白质、2 个 rRNA 和 57 个 tRNA 的基因。有趣的是，基因组分析揭示了参与 ATP 结合盒 (ABC) 转运蛋白依赖性 EPS 生物合成途径 ( ugd、ugd2、galU ) 的基因的存在。此外，我们还鉴定了与四氢嘧啶（ectA、ectB、ectC、ectD）和多羟基链烷酸酯（PHA；fabA、fabB、fabD、fabF、fabH、fabV、fabZ、phaC、phaD、phaG、phaR、phaZ1）有关的基因) 生产，已知其涉及盐环境中的细菌适应。这项研究的结果扩大了对海洋细菌参与 EPS 生物合成的基因和途径的科学认识。