BACKGROUND:Type two diabetes mellitus (T2DM) patients are prone to develop atherothrombotic events due to platelet hyper-reactivity stemming from platelet miRNA-223 down-regulation and over-expression of its corresponding target, P2RY12. OBJECTIVE:The study sought to determine the effects of long-term aerobic training on the expression levels of miRNA-223 and P2RY12 mRNA, and platelet function in T2DM patients. METHODS:Twenty-four patients with T2DM (age, 60.0±2.8 yrs.) were selected and randomly divided into two groups: aerobic exercise training (AET, n = 12) and control (CON, n = 12). The AET protocol was performed with moderate intensity for 12 weeks, while patients in the CON group followed their usual routine. Weight, body mass index (BMI), peak oxygen consumption (VO2peak), lipid profile, fasting blood glucose (FBG), glycated hemoglobin (HbA1c), insulin resistance index (HOMA-IR), platelet miRNA-223 and P2RY12 expression were measured before and after the period. RESULTS:There was a significant improvement in body weight, BMI, VO2peak, FBG, HbA1c, and HOMA-IR, after 12 weeks of AET (P < 0.01). Platelet aggregation decreased significantly after 12 weeks in the AET group compared with the CON (P < 0.001) group. Platelets’ miRNA-223 and P2RY12 were significantly up- and down-regulated after AET in comparison with the CON group (P < 0.05), respectively. Moreover, the relative expression of miRNA-223 and P2RY12 significantly correlated with FBG changes following the intervention. CONCLUSIONS:It can be concluded that long-term moderate-intensity aerobic training might be effective for reducing the occurrence of atherothrombotic events leading to premature death in T2DM patients through the modulation of miRNA-223, P2RY12 receptor expression, and platelet function.

中文翻译：

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2 型糖尿病患者的长期有氧运动训练改变 miRNA-223 及其相应靶点 P2RY12 受体的表达，减弱血小板功能

背景：2 型糖尿病（T2DM）患者由于血小板 miRNA-223 下调和其相应靶标 P2RY12 的过表达导致血小板高反应性，容易发生动脉粥样硬化血栓形成事件。目的：本研究旨在确定长期有氧训练对T2DM患者miRNA-223和P2RY12 mRNA表达水平及血小板功能的影响。方法：选择24例T2DM患者（年龄60.0±2.8岁），随机分为两组：有氧运动训练组（AET，n = 12）和对照组（CON，n = 12）。AET 方案以中等强度进行 12 周，而 CON 组的患者遵循他们的常规程序。体重、体重指数 (BMI)、峰值耗氧量 (VO2peak)、血脂、空腹血糖 (FBG)、期间测定糖化血红蛋白（HbA1c）、胰岛素抵抗指数（HOMA-IR）、血小板miRNA-223和P2RY12表达。结果：AET 12 周后，体重、BMI、VO2peak、FBG、HbA1c 和 HOMA-IR 均有显着改善（P < 0.01）。与 CON 组相比，AET 组 12 周后血小板聚集显着下降（P < 0.001）。与CON组相比，AET后血小板的miRNA-223和P2RY12分别显着上调和下调（P <0.05）。此外，干预后 miRNA-223 和 P2RY12 的相对表达与 FBG 变化显着相关。结论：