ABSTRACT

The root and aerial parts of Cotoneaster microphyllus were subjected to extraction and isolation of phytochemicals. The extracts were evaluated for their antioxidant anthelmintic, antimicrobial, and anticholinesterase potentials using standard protocols. Crude extract of aerial parts and roots, more potently scavenged DPPH free radicals with IC₅₀ values of 83 and 66 μg/mL while ABTS with 92 and 90 μg/mL respectively. Chloroform fraction exhibited highest anthelmintic activity followed by ethyl acetate fraction. Ethyl acetate fraction produced high zone of inhibition against selected bacterial and fungal strains. Maximum phenolic contents and vitamin C were found in the ethyl-acetate and chloroform fractions and were therefore, biologically the most potent fractions. Ethyl acetate fraction exhibited highest anticholinesterase potential and was therefore subjected to silica gel column chromatography which resulted in the isolation of one new (1) and four known (2–5) compounds. The isolated compounds were also screened for anticholinesterase potentials. Compound 3, most potently inhibited acetyl cholinesterase and butyryl cholinesterase with IC₅₀ value of 66 and 114 μg/mL, respectively. The extracts exhibited antioxidant, anticholinesterase, anthelmintic and antimicrobial potentials that should be further subjected for the isolation of other responsible compounds in pure state. As anticholinesterase compound 3 is a good candidate to be tested in animal models.

摘要

Cotoneaster microphyllus的根和地上部分进行了植物化学物质的提取和分离。使用标准方案评估提取物的抗蠕虫、抗微生物和抗胆碱酯酶潜力。地上部分和根的粗提取物，更有效地清除 DPPH 自由基，IC ₅₀值分别为 83 和 66 μg/mL，而 ABTS 分别为 92 和 90 μg/mL。氯仿部分表现出最高的驱虫活性，其次是乙酸乙酯部分。乙酸乙酯部分对选定的细菌和真菌菌株产生高抑制区。在乙酸乙酯和氯仿馏分中发现了最大的酚类含量和维生素 C，因此它们是生物学上最有效的馏分。乙酸乙酯部分表现出最高的抗胆碱酯酶潜力，因此进行硅胶柱层析，分离出一种新的 ( 1 ) 和四种已知的(2-5)化合物。还筛选了分离的化合物的抗胆碱酯酶潜力。化合物3，最有效地抑制乙酰胆碱酯酶和丁酰胆碱酯酶，IC ₅₀值分别为 66 和 114 μg/mL。提取物表现出抗氧化、抗胆碱酯酶、驱虫和抗微生物的潜力，应进一步分离纯态的其他相关化合物。作为抗胆碱酯酶，化合物3是在动物模型中进行测试的良好候选物。