Automated Optical Tweezers Manipulation to Transfer Mitochondria from Fetal to Adult MSCs to Improve Antiaging Gene Expressions,Small

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Automated Optical Tweezers Manipulation to Transfer Mitochondria from Fetal to Adult MSCs to Improve Antiaging Gene Expressions
Small ( IF 13.0 ) Pub Date : 2021-08-19 , DOI: 10.1002/smll.202103086
Adnan Shakoor ₁ , Bin Wang _{2,

3} , Lei Fan ₁ , Lingchi Kong ₃ , Wendi Gao ₁ , Jiayu Sun ₁ , Kwan Man ₄ , Gang Li _{2,

3} , Dong Sun ₁

Affiliation

Mitochondrial dysfunction is considered to be an important factor that leads to aging and premature aging diseases. Transferring mitochondria to cells is an emerging and promising technique for the therapy of mitochondrial deoxyribonucleic acid (mtDNA)-related diseases. This paper presents a unique method of controlling the quality and quantity of mitochondria transferred to single cells using an automated optical tweezer-based micromanipulation system. The proposed method can automatically, accurately, and efficiently collect and transport healthy mitochondria to cells, and the recipient cells then take up the mitochondria through endocytosis. The results of the study reveal the possibility of using mitochondria from fetal mesenchymal stem cells (fMSCs) as a potential source to reverse the aging-related phenotype and improve metabolic activities in adult mesenchymal stem cells (aMSCs). The results of the quantitative polymerase chain reaction analysis show that the transfer of isolated mitochondria from fMSCs to a single aMSC can significantly increase the antiaging and metabolic gene expression in the aMSC. The proposed mitochondrial transfer method can greatly promote precision medicine for cell therapy of mtDNA-related diseases.

中文翻译：

自动光镊操作将线粒体从胎儿转移到成人 MSC 以改善抗衰老基因表达

线粒体功能障碍被认为是导致衰老和早衰疾病的重要因素。将线粒体转移到细胞是治疗线粒体脱氧核糖核酸 (mtDNA) 相关疾病的一项新兴且有前景的技术。本文提出了一种使用基于自动光镊的显微操作系统控制转移到单个细胞的线粒体的质量和数量的独特方法。该方法可以自动、准确、高效地收集健康线粒体并将其运输到细胞中，然后受体细胞通过内吞作用摄取线粒体。研究结果揭示了使用胎儿间充质干细胞 (fMSCs) 的线粒体作为逆转衰老相关表型和改善成人间充质干细胞 (aMSCs) 代谢活动的潜在来源的可能性。定量聚合酶链反应分析的结果表明，将分离的线粒体从 fMSC 转移到单个 aMSC 可以显着增加 aMSC 中抗衰老和代谢基因的表达。所提出的线粒体转移方法可以极大地促进mtDNA相关疾病细胞治疗的精准医学。定量聚合酶链反应分析的结果表明，将分离的线粒体从 fMSC 转移到单个 aMSC 可以显着增加 aMSC 中抗衰老和代谢基因的表达。所提出的线粒体转移方法可以极大地促进mtDNA相关疾病细胞治疗的精准医学。定量聚合酶链反应分析的结果表明，将分离的线粒体从 fMSC 转移到单个 aMSC 可以显着增加 aMSC 中抗衰老和代谢基因的表达。所提出的线粒体转移方法可以极大地促进mtDNA相关疾病细胞治疗的精准医学。

更新日期：2021-09-23

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