The aims of the study were: (i) evaluate the efficacy of using amphibian adipose tissue as a valid biomarker of water contaminated by pyriproxyfen; and (ii) verify the use of Lithobates catesbeianus in laboratory experiments as a potential bioindicator for bioaccumulation of pesticide in adipose tissue from amphibians. Adult frogs were exposed to different dilutions (0.002 g/L and 0.02 g/L) of Sumilarv® (pyriproxyfen) over 50 days. The average results of the fortified sample were 108%, indicating that the test method was effective. Adult frogs exposed to the standard dose recommended by the World Health Organization (WHO) showed bioaccumulation of pyriproxyfen in adipose tissue significantly higher than control animals. Lithobates catesbeianus proved to be an effective bioindicator and the adipose tissue was an efficient biomarker to bioaccumulation of pyriproxyfen. We conclude that high-performance liquid chromatography–mass spectrometry was effective measuring pyriproxyfen bioaccumulation in adult amphibians.

该研究的目的是：(i) 评估使用两栖动物脂肪组织作为受吡丙醚污染的水的有效生物标志物的功效；(ii) 验证在实验室实验中使用Lithobates catesbeianus作为农药在两栖动物脂肪组织中生物积累的潜在生物指示剂。将成年青蛙暴露于不同稀释度（0.002 g/L 和 0.02 g/L）的 Sumilarv®（吡丙醚）中 50 天。强化样品的平均结果为 108%，表明该测试方法是有效的。暴露于世界卫生组织 (WHO) 推荐的标准剂量的成年青蛙在脂肪组织中的生物积累量显着高于对照动物。石斑鱼证明是有效的生物指示剂，脂肪组织是吡丙醚生物蓄积的有效生物标志物。我们得出结论，高效液相色谱-质谱法可有效测量成年两栖动物中吡丙醚的生物积累。