Nanotoxicity data for amphibians are limited compared to other taxonomic groups. The present study assessed toxicity of titanium dioxide nanoparticles (nanoTiO₂, anatase form, particle size < 25 nm) on tadpoles of Duttaphrynus melanostictus after short term and chronic exposures. Exposure to nanoTiO₂ (≤ 100 mg/L) for 96 h had no significant effect on survival but upon transfer to nanoTiO₂ free water for another 21 days, survival of the tadpoles pre-exposed to nanoTiO₂ was greatly reduced and their development was delayed. Chronic exposure to nanoTiO₂ (0.1 to 10 mg/L) for 14 days had no significant effects on acetylcholinesterase activities but induced mortalities (up to 40%) and histological alterations in the intestine and liver tissues. The results indicate that release of nanoTiO₂ to aquatic ecosystems could pose negative impacts to amphibian populations.

与其他分类群相比，两栖动物的纳米毒性数据有限。二氧化钛纳米颗粒的本研究评估毒性（nanoTiO ₂上的蝌蚪，锐钛矿形式，粒度<25纳米）黑眶蟾蜍短期和长期暴露后。暴露于nanoTiO ₂ (≤ 100 mg/L) 96 h 对存活没有显着影响，但转移到无纳米TiO _{2 的}水中再过21 天，预先暴露于nanoTiO ₂的蝌蚪的存活率大大降低，它们的发育延迟。长期暴露于 nanoTiO ₂(0.1 至 10 mg/L) 14 天对乙酰胆碱酯酶活性没有显着影响，但会导致死亡率（高达 40%）和肠和肝组织的组织学改变。结果表明，纳米TiO ₂释放到水生生态系统可能对两栖动物种群造成负面影响。