An intracellular leakage-trigged signal-on solid-state electrochemiluminescent (ECL) assay is developed for the detection of Escherichia coli (E. coli). A self-assembled multilayer ensemble of N, S co-doped carbon dots -poly dimethyl diallylammonium chloride grafted carbon nanospheres is used as ECL luminophores with peroxydisulfate (PS) ions as coreactants. The incorporation of molecularly imprinted electrospun nanofibers with the multilayer ensemble enables a robust, highly selective solid-state ECL probe without using any expensive and fragile biological receptor. Upon the imprinted E. coli exposed to the assay, under bactericidal effects of PS ions by destroying the integrity of E. coli cell membrane, intracellular leakage K⁺-triggered ECL enhancement is first disclosed via prompting the involved ¹O₂-mediated ECL process. Benefiting from the ECL enhancement upon increasing the concentration of E. coli, a unique intracellular leakage-trigged signal-on ECL system is created for sensing E. coli. Such a assay is proved to be highly specific and sensitive for sensing E. coli in the concentration range from 5 to 10⁷ cfu mL⁻¹, achieving a detection limit of 1 cfu mL⁻¹ (S/N = 3). This label-free, simple and facile assay provides a promising point-of-care diagnostic tool for pathogen detection.

开发了一种细胞内泄漏触发信号固态电化学发光 (ECL) 检测方法，用于检测大肠杆菌( E.coli )。N、S 共掺杂碳点-聚二甲基二烯丙基氯化铵接枝碳纳米球的自组装多层集合用作 ECL 发光体，过二硫酸盐 (PS) 离子作为共反应物。将分子印迹电纺纳米纤维与多层集合相结合，可实现稳健、高选择性的固态 ECL 探针，而无需使用任何昂贵且易碎的生物受体。在印迹大肠杆菌暴露于测定中后，在 PS 离子的杀菌作用下，通过破坏大肠杆菌细胞膜的完整性，细胞内渗漏 K^{+ -}触发的 ECL 增强首先是通过提示所涉及的¹ O ₂介导的 ECL 过程而公开的。受益于在增加大肠杆菌浓度时 ECL 增强，创建了一个独特的细胞内泄漏触发信号 ECL 系统，用于感应大肠杆菌。事实证明，这种测定对于检测浓度范围为 5 到 10 ⁷  cfu mL ^{-1 的}大肠杆菌具有高度特异性和敏感性，实现了 1 cfu mL ^-1 ( S / N  = 3)的检测限。这种无标记、简单易行的检测为病原体检测提供了一种很有前途的即时诊断工具。