The objective of this research was to investigate the α-glucosidase inhibitory activity of peptides released from Changii Radix protein under the optimal enzymatic hydrolysis conditions of trypsin, flavourzyme, compound proteinase, papain, alkaline proteinase, neutral protease, and in vitro simulated human gastrointestinal digestive conditions. The hydrolysate with the best activity was further purified by ultrafiltration, size-exclusion chromatography and semi-preparative high performance liquid chromatography. The peptides in the most active fraction were subsequently identified by nano-LC–MS/MS and screened for bioactivity. As a result, the GID-3-SG-2-RP-2 fraction (with smaller molecular weight and more hydrophobic) manifested the highest effect with an IC₅₀ value of 32 μg/mL. Three peptides of this fraction were synthesized, SQHISTAGMEASGTSNMKF, KVIISAPSKDAPMF, and STFQQMW, and provided an IC₅₀ of 1.051, 0.032, and 1.104 mg/mL, respectively. Thus, the results indicated that Changii Radix derived peptides may have potential functions in the prevention and management of type 2 diabetes.

本研究的目的是研究在胰蛋白酶、风味酶、复合蛋白酶、木瓜蛋白酶、碱性蛋白酶、中性蛋白酶和体外模拟人胃肠道消化的最佳酶解条件下樟宜蛋白释放肽的α-葡萄糖苷酶抑制活性。状况。通过超滤、尺寸排阻色谱和半制备高效液相色谱进一步纯化具有最佳活性的水解产物。随后通过纳米 LC-MS/MS 鉴定最活跃部分中的肽并筛选生物活性。因此，GID-3-SG-2-RP-2 级分（分子量更小，疏水性更强）表现出最高的效果，IC ₅₀值 32 μg/mL。合成了该级分的三种肽，SQHISTAGMEASGTSNMKF、KVIISAPSKDAPMF 和 STFQQMW，分别提供了 1.051、0.032和 1.104 mg/mL的 IC ₅₀。因此，结果表明，樟宜提取的多肽在预防和管理 2 型糖尿病方面可能具有潜在的功能。