A new inactivated vaccine against Bluetongue virus (BTV) serotypes 1 and 4, was developed from field isolates. Safety and efficacy of the vaccine were evaluated in sheep by serological monitoring and virus nucleic acid detection after experimental infection of vaccinated animals. Seroconversion was observed in vaccinated animals at day 14 post vaccination (pv) with neutralizing antibody titer of 1.9 and 1.8 for serotypes 1 and 4, respectively. The titer increase significantly after the booster reaching 2.7 and persist one year >1.5 for both serotypes. After challenge with virulent isolates, vireamia was recorded in control animals, as evident by q-PCR with threshold cycles (Ct) ranging from 24 to 31 and peaked at day 10 post challenge, while no vireamia was detected in vaccinated animals. Vaccinated sheep were fully protected against the disease and infection.

一种针对蓝舌病毒 (BTV) 血清型 1 和 4 的新型灭活疫苗是从野外分离物中开发出来的。通过对接种动物进行实验感染后的血清学监测和病毒核酸检测，在绵羊中评估疫苗的安全性和有效性。在接种疫苗 (pv) 后第 14 天，在接种疫苗的动物中观察到血清转化，血清型 1 和 4 的中和抗体滴度分别为 1.9 和 1.8。两种血清型在加强剂达到 2.7 后滴度显着增加并持续一年 >1.5。用毒力分离株攻击后，在对照动物中记录了病毒血症，正如 q-PCR 所证明的那样，阈值循环 (Ct) 范围从 24 到 31，并在攻击后第 10 天达到峰值，而在接种疫苗的动物中未检测到病毒血症。