In this study, Lactobacillus crispatus UBLCp01, Lactobacillus gasseri UBLG36, and Lactobacillus johnsonii UBLJ01 isolated from the vagina of healthy reproductive age Indian women were screened for beneficial probiotic properties. These strains showed the ability to survive acidic and simulated vaginal fluid conditions and could adhere to mucin. Lact. gasseri UBLG36, and Lact. johnsonii UBLJ01 produced d- and l-lactic acid, whereas Lact. crispatus UBLCp01 produced hydrogen peroxide and d- and l-lactic acid. All strains inhibited the growth of pathogens (Escherichia coli, Gardnerella vaginalis, Proteus mirabilis, and Candida albicans) and were capable of co-aggregating with them with varying degrees. Strains secreted exopolysaccharides and formed biofilms under in vitro conditions. Safety assessment showed that these strains had a usual antibiotic susceptibility profile, did not produce hemolysins, gelatinases, and mucin degrading enzymes. Based on strain characteristics and beneficial properties, we believe that these strains are promising candidates for human trials to confirm their ability to prevent/treat vaginal dysbiosis and maintain a healthy vaginal eco-system.

在这项研究中，从印度健康育龄妇女的阴道中分离出的卷曲乳杆菌UBLCp01、加氏乳杆菌UBLG36 和约氏乳杆菌UBLJ01 被筛选出有益的益生菌特性。这些菌株显示出在酸性和模拟的阴道液条件下存活的能力，并且可以粘附到粘蛋白上。乳汁。gasseri UBLG36 和Lact。johnsonii UBLJ01 产生d - 和l - 乳酸，而Lact。crispatus UBLCp01 产生过氧化氢和d - 和l - 乳酸。所有菌株均抑制病原体的生长（大肠杆菌、阴道加德纳菌、奇异变形杆菌和白色念珠菌），并能不同程度地与它们共聚集。菌株在体外条件下分泌胞外多糖并形成生物膜。安全评估表明，这些菌株具有常见的抗生素敏感性特征，不产生溶血素、明胶酶和粘蛋白降解酶。基于菌株特征和有益特性，我们认为这些菌株是有前途的人体试验候选者，以确认它们预防/治疗阴道生态失调和维持健康阴道生态系统的能力。