The complete genome sequence of a Brazilian isolate of yambean mosaic virus (YBMV) is presented. High-throughput sequencing (Illumina HiSeq) and Sanger sequencing revealed the complete genome sequence of the YBMV-BRA-6 isolate, found in Canavalia ensiformis. The de novo contigs were assembled into a 9612 nucleotides (nt) long scaffold, excluding the 3’-terminal poly(A) tail, covering the complete genome. The genomic RNA contains an open reading frame (ORF) typical of members of the genus Potyvirus, family Potyviridae, encoding a large putative polyprotein of 3078 amino acids (aa) and a small overlapping PIPO ORF. Pairwise comparisons showed that the YBMV-BRA-6 isolate sequence shares 88.1% nt identity for the complete genome and 90.6% aa identity for the polyprotein with the YBMV-SR isolate. Phylogenetic analysis grouped both isolates together and close to bean common mosaic virus (BCMV). The polyprotein cleavage sites were predicted and a recombination event is described.

介绍了巴西豆花叶病毒 (YBMV) 分离株的完整基因组序列。高通量测序 (Illumina HiSeq) 和 Sanger 测序揭示了在Canavalia ensiformis中发现的 YBMV-BRA-6 分离株的完整基因组序列。de novo contigs 组装成一个 9612 个核苷酸 (nt) 长的支架，不包括 3'-末端 poly(A) 尾，覆盖整个基因组。基因组 RNA 包含一个开放阅读框 (ORF)，该开放阅读框 (ORF) 是马铃薯病毒属、马铃薯病毒科成员的典型特征。，编码 3078 个氨基酸 (aa) 的大型假定多蛋白和一个小的重叠 PIPO ORF。成对比较表明，YBMV-BRA-6 分离物序列与 YBMV-SR 分离物的完整基因组具有 88.1% 的 nt 同一性，而多蛋白的 aa 同一性为 90.6%。系统发育分析将这两种分离株归为一类，并与豆类常见花叶病毒 (BCMV) 接近。预测了多蛋白切割位点并描述了重组事件。