Introduction: The concentration of 25-hydroxycholecalciferol (25OHD₃) in the serum of obese people is low and often accompanied by symptoms of low fertility. Therefore, vitamin D is recommended as a potential treatment option. However, after clinical trials, it was found that vitamin D cannot effectively increase the concentration of 25OHD₃ in the serum of obese people. How obesity causes low 25OHD₃ concentration and low fertility is unclear. Methods: We analyzed the physiological and pathological changes in obese mice induced by a high-fat diet (HFD) and the changes in mice after supplementing with 25OHD₃. Results: The concentration of 25OHD₃ in the serum of obese mice induced by HFD was significantly reduced, and these mice showed liver hypertrophy accompanied by abnormal liver injury, testicular hypertrophy, low testosterone levels, high leptin levels, and low sperm motility. The mRNA and protein expression of CYP2R1 of hydroxylated vitamin D₃ was significantly reduced; CYP11A1 and CYP11A2, which synthesize testosterone, were significantly reduced. After supplementing with 25OHD₃, there was an increase in serum 25OHD₃ concentration, testosterone level, and sperm motility, but it cannot improve the degree of obesity, CYP2R1 expression, and liver damage. Conclusion: Our research shows that there is a metabolic interference mediated by 25OHD₃ and testosterone between obesity and low sperm motility. The results of this study can provide a scientific basis for studying the mechanism of 25OHD₃ and hormone regulation and treating obese people with low sperm motility.
Obes Facts

中文翻译：

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肥胖小鼠血清 25OHD3 受肝损伤的影响并与睾酮水平和精子活力相关

简介：肥胖者血清中25-羟基胆钙化醇（25OHD ₃）浓度偏低，常伴有生育力低下的症状。因此，建议将维生素 D 作为潜在的治疗选择。但经过临床试验发现，维生素D并不能有效提高肥胖人群血清中25OHD ₃的浓度。肥胖如何导致低 25OHD ₃浓度和低生育能力尚不清楚。方法：我们分析了高脂饮食（HFD）引起的肥胖小鼠的生理和病理变化以及补充25OHD ₃后小鼠的变化。结果： 25OHD ₃的浓度HFD 诱导的肥胖小鼠血清中的 α 显着降低，这些小鼠表现为肝肥大并伴有异常肝损伤、睾丸肥大、睾酮水平低、瘦素水平高、精子活力低。羟基化维生素D ₃ CYP2R1 mRNA和蛋白表达显着降低；合成睾酮的 CYP11A1 和 CYP11A2 显着减少。补充25OHD _{3 后}，血清25OHD ₃浓度、睾酮水平、精子活力增加，但不能改善肥胖程度、CYP2R1表达和肝损害。结论：我们的研究表明存在由 25OHD ₃介导的代谢干扰和睾酮之间的肥胖和低精子活力。本研究结果可为研究25OHD ₃与激素调节机制及治疗肥胖者精子活力低下提供科学依据。
肥胖事实