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Antiproliferative effects of Turkish pomegranate (Punica granatum L.) extracts on MCF-7 human breast cancer cell lines with focus on antioxidant potential and bioactive compounds analyzed by LC-MS/MS
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2021-08-19 , DOI: 10.1111/jfbc.13904 Esra Eroglu Ozkan 1 , Mehmet Fatih Seyhan 2 , Ozlem Kurt Sirin 3 , Tugba Yilmaz-Ozden 3 , Ezgi Ersoy 1, 4 , Seda Damla Hatipoglu Cakmar 5 , Ahmet Ceyhan Goren 5, 6 , Hulya Yilmaz Aydogan 7 , Oguz Ozturk 7
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2021-08-19 , DOI: 10.1111/jfbc.13904 Esra Eroglu Ozkan 1 , Mehmet Fatih Seyhan 2 , Ozlem Kurt Sirin 3 , Tugba Yilmaz-Ozden 3 , Ezgi Ersoy 1, 4 , Seda Damla Hatipoglu Cakmar 5 , Ahmet Ceyhan Goren 5, 6 , Hulya Yilmaz Aydogan 7 , Oguz Ozturk 7
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In this study, eight different pomegranate (Punica granatum L.) cultivars from Turkey were evaluated for their antioxidant and cytotoxic effects on the MCF-7 breast cancer cell lines and MCF-10A breast fibrocystic epithelial cell lines with a focus on their chemical compositions by LC-MS/MS. Cell lines were treated with pomegranate juice extracts in different doses at selected time intervals (24th, 48th, and 72nd hour). Afterwards, WST-1 cell proliferation assay was performed to investigate the cytotoxicity of the extracts. Accordingly, all extracts decreased the cell viability of MCF-7 breast cancer cell lines and had no cytotoxic effect on the cell viability of MCF-10A cell lines. Among eight extracts, P7 (Izmir 1513), which was rich in anthocyanins such as cyanidin chloride (69.76 ± 8.02 μg/g extract), cyanidin-3-O-glucoside (903.66 ± 101.89 μg/g extract), and punicalagin (992.09 ± 174.53 μg/g extract), was found to demonstrate the strongest cytotoxic activity on MCF-7 breast cancer cell lines by decreasing the cell viability in half at 24th hour with an IC50 value of 49.08 µg/ml.
中文翻译：
土耳其石榴 (Punica granatum L.) 提取物对 MCF-7 人乳腺癌细胞系的抗增殖作用，重点是通过 LC-MS/MS 分析的抗氧化潜力和生物活性化合物
在这项研究中，八种不同的石榴 ( Punica granatumL.) 评估了来自土耳其的栽培品种对 MCF-7 乳腺癌细胞系和 MCF-10A 乳腺纤维囊性上皮细胞系的抗氧化和细胞毒性作用，重点是通过 LC-MS/MS 评估它们的化学成分。在选定的时间间隔（第 24、48 和 72 小时）用不同剂量的石榴汁提取物处理细胞系。然后，进行 WST-1 细胞增殖试验以研究提取物的细胞毒性。因此，所有提取物都降低了 MCF-7 乳腺癌细胞系的细胞活力，并且对 MCF-10A 细胞系的细胞活力没有细胞毒性作用。在八种提取物中，P7 (Izmir 1513) 富含花青素，如氯化花青素（69.76 ± 8.02 μg/g 提取物）、花青素-3-O-葡萄糖苷（903.66 ± 101.89 μg/g 提取物）和安石榴苷（992.09） ± 174.53 μg/g 提取物），50值为 49.08 µg/ml。
更新日期：2021-09-06
中文翻译：
土耳其石榴 (Punica granatum L.) 提取物对 MCF-7 人乳腺癌细胞系的抗增殖作用，重点是通过 LC-MS/MS 分析的抗氧化潜力和生物活性化合物
在这项研究中，八种不同的石榴 ( Punica granatumL.) 评估了来自土耳其的栽培品种对 MCF-7 乳腺癌细胞系和 MCF-10A 乳腺纤维囊性上皮细胞系的抗氧化和细胞毒性作用，重点是通过 LC-MS/MS 评估它们的化学成分。在选定的时间间隔（第 24、48 和 72 小时）用不同剂量的石榴汁提取物处理细胞系。然后，进行 WST-1 细胞增殖试验以研究提取物的细胞毒性。因此，所有提取物都降低了 MCF-7 乳腺癌细胞系的细胞活力，并且对 MCF-10A 细胞系的细胞活力没有细胞毒性作用。在八种提取物中，P7 (Izmir 1513) 富含花青素，如氯化花青素（69.76 ± 8.02 μg/g 提取物）、花青素-3-O-葡萄糖苷（903.66 ± 101.89 μg/g 提取物）和安石榴苷（992.09） ± 174.53 μg/g 提取物），50值为 49.08 µg/ml。
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