Allium jesdianum Boiss. & Buhse. is the most important species of the Amaryllidaceae family with various pharmacological properties. Three subsequent experiments (germination, callogenesis, and elicitation) were carried out as a completely randomized design with six replication. At the first study, the highest seed germination (78.33%) was achieved at chemical pre-treatment including the combination of α-naphthalene acetic acid (1 mg L⁻¹) and benzylaminopurine (3 mg L⁻¹) under in vitro condition. The highest callus induction (86.7%) was observed at MS/2 media, which was supplemented by NAA (1 mg L⁻¹) and BAP (3 mg L⁻¹) from hypocotyl explants. Then, two chemical elicitors including methyl jasmonate (MeJ) (0, 25, 50, and 100 µM) and putrescine (Pu) (0, 0.5, and 1 mM) were used to investigate their effects on different biochemical traits under callus culture. The results showed the superiority of MeJ over Pu for increasing the secondary metabolites and antioxidant activity in calluses of Allium jesdianum, compared to the control. The highest contents for total phenolics (6.02 mg GAE g⁻¹ FW), total flavonoids (0.52 mg QE g⁻¹ FW), and total flavonols (0.39 mg QE g⁻¹ FW) were observed under 50 µM of MeJ. Meanwhile, the highest value for anthocyanin (8.99 µ mol g⁻¹ FW) was achieved at 25 µM of MeJ. The highest 2,2-diphenyl-1-picrylhydrazyl activities were observed at 50 and 100 µM of MeJ. Putrescine (0.5 mM) elicitation showed only superiority for callus growth rate (0.53 mm day⁻¹). Enhancement of desired secondary metabolites at 50 µM MeJ could be suitable for future studies in biotechnological aspects of this medicinal plant.

葱属 jesdianum Boiss。 ＆布什。是石蒜科最重要的物种，具有多种药理特性。三个后续实验（萌发、愈伤组织和诱导）以完全随机设计的方式进行，并进行了六次重复。在第一项研究中，在体外条件下，化学预处理（包括α-萘乙酸（1 mg L ^-1 ）和苄氨基嘌呤（3 mg L ^-1 ）组合）实现了最高的种子发芽率（78.33％）。在补充有来自下胚轴外植体的NAA (1 mg L ^-1 )和BAP (3 mg L ^-1 )的MS/2培养基中观察到最高的愈伤组织诱导(86.7%)。然后，使用茉莉酸甲酯（MeJ）（0、25、50和100 µM）和腐胺（Pu）（0、0.5和1 mM）两种化学诱导子研究它们对愈伤组织培养下不同生化性状的影响。结果表明，与对照相比，MeJ 在增加葱愈伤组织中次生代谢产物和抗氧化活性方面优于 Pu。在50 µM MeJ下观察到总酚类（6.02 mg GAE g ^-1 FW）、总黄酮（0.52 mg QE g ^-1 FW）和总黄酮醇（0.39 mg QE g ^-1 FW）的最高含量。同时，花青素的最高值（8.99 µ mol g ^-1 FW）在 25 µM MeJ 下实现。在 50 和 100 µM MeJ 下观察到最高的 2,2-二苯基-1-三硝基苯肼活性。腐胺(0.5 mM)诱导仅显示出愈伤组织生长速率的优势(0.53 mm day ^-1 )。 在 50 µM MeJ 下增强所需的次生代谢物可能适合这种药用植物生物技术方面的未来研究。