A bifunctional glycoside hydrolase GH78 from the ascomycete Xylaria polymorpha (XpoGH78) possesses the catalytic versatility towards both glycosides and esters, which may be advantageous for the efficient degradation of the plant cell-wall complex that contains both diverse sugar residues and esterified structures. The contribution of XpoGH78 to the conversion of lignocellulosic materials without any chemical pretreatment to release the water-soluble aromatic fragments, carbohydrates, and methanol was studied. The disintegrating effect of enzymatic lignocellulose treatment can be significantly improved by using different kinds of hydrolases and phenoloxidases. The considerable changes in low (3 kDa), medium (30 kDa), and high (> 200 kDa) aromatic fragments were observed after the treatment with XpoGH78 alone or with this potent cocktail. Synergistic conversion of rape straw also resulted in a release of 17.3 mg of total carbohydrates (e.g., arabinose, galactose, glucose, mannose, xylose) per gram substrate after incubating for 72 hrs. Moreover, the treatment of rape straw with XpoGH78 led to a marginal methanol release of approx. 17 µg g^-1 and improved to 270 µg g^-1 by cooperation with the above accessory enzymes. In the case of beech-wood conversion, the combined catalysis by XpoGH78 and laccase caused a comparable effect concerning the liberation of aromatic lignocellulose fragments as fungal strain X. polymorpha in woody cultures did.

中文翻译：

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利用多形木聚糖的多功能 GH78 糖苷水解酶对木质纤维素材料进行生物转化，释放芳香片段和碳水化合物。


来自子囊菌Xylaria polymorpha的双功能糖苷水解酶 GH78 ( Xpo GH78) 具有对糖苷和酯的催化多功能性，这可能有利于有效降解含有多种糖残基和酯化结构的植物细胞壁复合物。研究了Xpo GH78 对木质纤维素材料转化的贡献，无需任何化学预处理即可释放水溶性芳香族片段、碳水化合物和甲醇。使用不同种类的水解酶和酚氧化酶可以显着提高酶法木质纤维素处理的崩解效果。单独使用Xpo GH78 或使用这种强效混合物处理后，观察到低 (3 kDa)、中 (30 kDa) 和高 (> 200 kDa) 芳香片段的显着变化。培养72小时后，油菜秸秆的协同转化还导致每克底物释放17.3毫克总碳水化合物（例如阿拉伯糖、半乳糖、葡萄糖、甘露糖、木糖）。此外，用Xpo GH78 处理油菜秸秆导致甲醇边际释放量约为。 17 µg g ^-1并通过与上述辅助酶的配合提高至270 µg g ^-1 。在山毛榉木转化的情况下， Xpo GH78 和漆酶的联合催化作用在释放芳香木质纤维素片段方面产生了与木本培养物中的真菌菌株X. polymorpha类似的效果。