Heat stress in poultry is deleterious to productive performance. Chlorogenic acid (CGA) exerts antibacterial, anti-inflammatory, and antioxidant properties. This study was conducted to evaluate the effects of dietary supplemental CGA on the intestinal health and cecal microbiota composition of young hens challenged with acute heat stress. 100-day-old Hy-line brown pullets were randomly divided into four groups. The control group (C) and heat stress group (HS) received a basal diet. HS + CGA₃₀₀ group and HS + CGA₆₀₀ group received a basal diet supplemented with 300- and 600-mg/kg CGA, respectively, for 2 weeks before heat stress exposure. Pullets of HS, HS + CGA₃₀₀, and HS + CGA₆₀₀ group were exposed to 38°C for 4 h while the control group was maintained at 25°C. In this study, dietary CGA supplementation had effect on mitigate the decreased T-AOC and T-SOD activities and the increasing of IL-1β and TNFα induced by acute heat stress. Dietary supplementation with 600 mg/kg CGA had better effect on increasing the relative abundance of beneficial bacterial genera, such as Rikenellaceae RC9_gut_group, Ruminococcaceae UCG-005, and Christensenellaceae R-7_group, and deceasing bacteria genera involved in inflammation, such as Sutterella species. Therefore, CGA can ameliorate acute heat stress damage through suppressing inflammation and improved antioxidant capacity and cecal microbiota composition.

中文翻译：

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绿原酸对急性热应激幼母鸡肠道炎症、抗氧化状态和微生物群落的影响

家禽的热应激对生产性能有害。绿原酸 (CGA) 具有抗菌、抗炎和抗氧化特性。本研究旨在评估日粮补充 CGA 对受到急性热应激挑战的幼母鸡的肠道健康和盲肠微生物群组成的影响。将 100 日龄的 Hy-line 棕色后备母鸡随机分为四组。对照组 (C) 和热应激组 (HS) 接受基础饮食。HS + CGA ₃₀₀组和 HS + CGA ₆₀₀组分别在热应激暴露前 2 周接受基础饮食补充 300 和 600 mg/kg CGA。HS、HS + CGA ₃₀₀和 HS + CGA _{600的小母鸡}组暴露于 38°C 4 h，而对照组保持在 25°C。在这项研究中，膳食 CGA 补充剂对减轻急性热应激诱导的T-AOC 和 T-SOD 活性降低以及IL-1β和TNFα升高有影响。膳食补充 600 mg/kg CGA 对增加有益细菌属的相对丰度有更好的效果，例如Rikenellaceae RC9_gut_group、Ruminococcaceae UCG-005和Christensenellaceae R-7_group，并减少与炎症有关的细菌属，例如Sutterella物种。因此，CGA 可以通过抑制炎症、改善抗氧化能力和盲肠微生物群组成来改善急性热应激损伤。