Abstract—

DyeCycle Violet efflux, caused by ATP-binding cassette transporters activity, was analyzed in human colorectal adenocarcinoma cell lines SW480, HT-29, Caco-2 by means of a FACSAria III flow cytometer and an ImageStreamX Mk II imaging flow cytometer. Besides similarity of cytometry data obtained by two instruments, the use of imaging flow cytometry made it possible to characterize the morphology of side population cells, as well as morphology of other cell populations differing in the degree of dye accumulation. The population of cells, which are smaller than the side population cells and practically do not take the dye, is of the special interest. Probably, this population may contribute to the tumor resistance to chemotherapy.

中文翻译：

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使用成像流式细胞术鉴定与 ATP 结合盒转运蛋白活性相关的侧群

摘要——

借助 FACSAria III 流式细胞仪和 ImageStreamX Mk II 成像流式细胞仪，在人结直肠腺癌细胞系 SW480、HT-29、Caco-2 中分析了由 ATP 结合盒转运蛋白活性引起的 DyeCycle Violet 流出。除了通过两种仪器获得的细胞计数数据的相似性外，成像流式细胞术的使用还可以表征侧群细胞的形态，以及其他染料积累程度不同的细胞群的形态。细胞群比侧群细胞小并且实际上不带染料，是特别令人感兴趣的。可能，这个群体可能有助于肿瘤对化疗的耐药性。