Abstract

We analyzed the distribution of the ordering parameter of raft-like microdomains in the mitochondrial membranes of halophytes, which differ in the type of salt tolerance: Salicornia perennans, Halocnemum strobilaceum, and Artemisia santonica. The packing density of the membrane lipids (lipid order) was assessed by measuring generalized polarization (GP) of the Laurdan fluorescence. In the mitochondrial membrane material of S. perennans, H. strobilaceum, and A. santonica, in the range of 15 and 25% of the sucrose density gradient, the GP distributions contain 4–6 components presumably related to the raft structures. Lipids isolated from ordered domains are enriched in sterols, cerebrosides, and saturated fatty acids; the quantitative content of raft-forming lipids depends on the salt tolerance strategy of halophytes.

中文翻译：

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荧光显微镜下盐生植物线粒体膜筏状结构的脂质排列分析

摘要

我们分析了盐生植物线粒体膜中筏状微区的排序参数的分布，这些植物在耐盐性类型上有所不同：长春花、Halocnemum strobilaceum和Artemisia santonica。通过测量 Laurdan 荧光的广义极化 (GP) 来评估膜脂质的堆积密度（脂质顺序）。在S. perennans、H. strobilaceum和A. santonica的线粒体膜材料中，在蔗糖密度梯度的 15% 和 25% 范围内，GP 分布包含 4-6 个可能与筏结构相关的分量。从有序结构域分离的脂质富含甾醇、脑苷脂和饱和脂肪酸；形成筏的脂质的定量含量取决于盐生植物的耐盐策略。