Cervical cancer is a common female malignancy worldwide, and the molecular mechanism of cervical tumorigenesis remains poorly understood. A large piece of evidence have demonstrated the important roles of long noncoding RNAs (lncRNAs) in tumorigenesis, cancer progression and drug resistance. In this study, we comprehensively analyzed the lncRNAs expression pattern in cervical cancer using RNA sequencing and microarray data from the cancer genome atlas, gene expression omnibus and Genotype Tissue Expression. Moreover, we assessed the correlation between lncRNA expression levels and cervical cancer patient's survival. We uncovered hundreds of lncRNAs that are upregulated or downregulated in cervical cancer tissues. Among these aberrantly lncRNAs, some are significantly associated with cervical patients' poorer prognosis, such as ALOX12-AS1 and LINC00173. ALOX12-AS1 expression is downregulated in cervical cancer, and over-expression of ALOX12-AS1 could inhibit cervical cancer cells proliferation in vitro. Further, mechanistically investigation revealed that ALOX12-AS1 could interact with AGO2 and sponge miR-3171, thereby antagonizing its' repression of tumor suppressor phosphatase and tensin homolog expression in cervical cancer cell. Taken together, this study provides lncRNA candidates in cervical cancer and highlights the critical role of ALOX12-AS1 in cervical cancer.

中文翻译：

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长非编码 RNA ALOX12-AS1 通过靶向 miR-3171 抑制宫颈癌细胞增殖。

宫颈癌是世界范围内常见的女性恶性肿瘤，而宫颈肿瘤发生的分子机制仍知之甚少。大量证据证明了长链非编码RNA（lncRNA）在肿瘤发生、癌症进展和耐药性中的重要作用。在这项研究中，我们利用来自癌症基因组图谱、基因表达综合和基因型组织表达的RNA测序和微阵列数据，全面分析了宫颈癌中的lncRNA表达模式。此外，我们评估了lncRNA表达水平与宫颈癌患者生存率之间的相关性。我们发现了数百种在宫颈癌组织中上调或下调的 lncRNA。在这些异常的lncRNA中，有些与宫颈患者较差的预后显着相关，例如ALOX12-AS1和LINC00173。ALOX12-AS1在宫颈癌中表达下调，过表达ALOX12-AS1可以抑制体外宫颈癌细胞的增殖。此外，机制研究表明，ALOX12-AS1 可以与 AGO2 和海绵 miR-3171 相互作用，从而拮抗其对宫颈癌细胞中抑癌磷酸酶和张力蛋白同源物表达的抑制。总而言之，这项研究提供了宫颈癌中的 lncRNA 候选者，并强调了 ALOX12-AS1 在宫颈癌中的关键作用。