Due to a lack of data on predictors of electroporation-based treatment outcomes, we investigated the potential predictive role of contrast-enhanced harmonic ultrasound (CEUS) in mice B16F10 melanoma treated by gene electrotransfer (GET) to silence melanoma cell adhesion molecule (MCAM) and radiotherapy, which has not been evaluated yet. CEUS evaluation was verified by tumor histological analysis. Mice bearing subcutaneous tumors were treated with GET to silence MCAM, irradiation or the combination of GET to silence MCAM and irradiation (combined treatment). CEUS of the tumors used to evaluate tumor perfusion was performed before and up to 10 days after the beginning of the experiment, and the CEUS results were compared with tumor growth and the number of blood vessels analyzed in the histological tumor sections. CEUS revealed a decrease in tumor perfusion in the combined therapy groups compared with the control groups and correlated with tumor histological analyses, which showed a decreased vascular density. In this study a trend of inverse correlation was observed between tumor perfusion and treatment efficacy. The greater the perfusion of the tumor, the shorter the expected doubling time. Furthermore, decreased perfusion showed a trend to correlate with higher antitumor efficacy. Thus, CEUS could be used to predict tumoral vascular density and treatment effectiveness.

由于缺乏基于电穿孔的治疗结果的预测因子的数据，我们研究了对比增强谐波超声 (CEUS) 在通过基因电转移 (GET) 治疗的小鼠 B16F10 黑色素瘤中对沉默黑色素瘤细胞粘附分子 (MCAM) 的潜在预测作用和尚未评估的放射疗法。CEUS评估通过肿瘤组织学分析得到验证。带有皮下肿瘤的小鼠用 GET 治疗以使 MCAM 沉默、辐射或 GET 的组合以使 MCAM 和辐射沉默（联合治疗）。用于评估肿瘤灌注的肿瘤的 CEUS 在实验开始前和开始后 10 天进行，并将 CEUS 结果与组织学肿瘤切片中分析的肿瘤生长和血管数量进行比较。CEUS 显示，与对照组相比，联合治疗组的肿瘤灌注减少，并且与肿瘤组织学分析相关，后者显示血管密度降低。在这项研究中，观察到肿瘤灌注和治疗效果之间呈负相关的趋势。肿瘤的灌注越大，预期的倍增时间越短。此外，灌注减少显示出与更高的抗肿瘤功效相关的趋势。因此，CEUS 可用于预测肿瘤血管密度和治疗效果。肿瘤的灌注越大，预期的倍增时间越短。此外，灌注减少显示出与更高的抗肿瘤功效相关的趋势。因此，CEUS 可用于预测肿瘤血管密度和治疗效果。肿瘤的灌注越大，预期的倍增时间越短。此外，灌注减少显示出与更高的抗肿瘤功效相关的趋势。因此，CEUS 可用于预测肿瘤血管密度和治疗效果。