In this work, effects of particle and ligand types on retention and separation of sets of neutral (naphthoflavones), acidic (profens), basic (cathinones), and zwitterionic (dipeptides) biologically active compounds are shown in detail and discussed. Nine columns differing in ligand and particle types were tested, namely XSelect HSS C18, XSelect HSS C18 SB, XSelect HSS PFP, XSelect CSH C18, XSelect CSH Phenyl-Hexyl, XSelect CSH Fluoro-Phenyl, XBridge C18, XBridge Shield RP18, and Atlantis PREMIER BEH C18 AX. Various mobile phases differing in acetonitrile/aqueous part volume ratio and aqueous part pH, i.e. pH = 2.1, pH = 4.7, and pH = 8.0 were tested and compared in terms of analytes´ retention and separation. Results showed significant effect of ionic interactions resulting from particle types used or additional ionisable functional groups bonded to the surface on retention and separation of charged compounds. All tested sets of compounds were baseline separated but different conditions, that is column type and mobile phase composition, were needed.

Graphic abstract

中文翻译：

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配体和颗粒类型如何影响 HPLC 中结构多样的生物活性化合物的保留和分离

在这项工作中，详细显示和讨论了颗粒和配体类型对中性（萘黄酮）、酸性（profens）、碱性（卡西酮）和两性离子（二肽）生物活性化合物组的保留和分离的影响。测试了九种不同配体和颗粒类型的色谱柱，即 XSelect HSS C18、XSelect HSS C18 SB、XSelect HSS PFP、XSelect CSH C18、XSelect CSH Phenyl-Hexyl、XSelect CSH Fluoro-Phenyl、XBridge C18、XBridge Shield RP18 和 Atlantis高级 BEH C18 AX。在乙腈/水部分体积比和水部分 pH 值（即 pH = 2.1、pH = 4.7 和 pH = 8.0）方面不同的各种流动相在分析物的保留和分离方面进行了测试和比较。结果表明，所使用的颗粒类型或键合到表面的附加可电离官能团产生的离子相互作用对带电化合物的保留和分离有显着影响。所有测试的化合物组都进行了基线分离，但需要不同的条件，即色谱柱类型和流动相组成。

图形摘要