Trifolium (Fabaceae) is widely distributed with more than 255 species. The current study contributed to the knowledge of the diversity and genetic population structure of eight Iranian Trifolium, which remained relatively obscure. The taxonomic circumscription of sections and varieties is still questionable and poorly understood. The current study contributed to the genetic diversity, and population genetics of eight Iranian Trifolium specimens belonging to sections Mistyllus and Vesicaria based on IRAP and SSR datasets. A considerable level of polymorphism was detected among specimens. Molecular variance analysis exhibited much more genetic variation within populations in IRAP genetic marker, while an equal level of variation was detected within and among populations in SSR. IRAP and SSR genetic markers exhibited significant tree incongruence mainly due to the existence of a different genomic constitution. Furthermore, the results confirmed that the Vesicaria and Mistyllus sections are completely separate.

三叶草（豆科）分布广泛，有超过 255 种。目前的研究有助于了解八种伊朗三叶草的多样性和遗传种群结构，这些知识仍然相对模糊。部分和品种的分类范围仍然有问题且知之甚少。目前的研究为属于Mistyllus和Vesicaria部分的八个伊朗三叶草标本的遗传多样性和种群遗传学做出了贡献基于 IRAP 和 SSR 数据集。在标本中检测到相当程度的多态性。分子方差分析在 IRAP 遗传标记中的种群内表现出更多的遗传变异，而在 SSR 中种群内和种群之间检测到相同水平的变异。IRAP 和 SSR 遗传标记表现出显着的树不一致，主要是由于存在不同的基因组构成。此外，结果证实Vesicaria和Mistyllus部分是完全分开的。