Rhabdomyolysis is a clinical syndrome caused by damage to skeletal muscle, which consequently releases breakdown products into circulation and causes acute kidney injury (AKI) in humans. Intramuscular injection of glycerol mimics rhabdomyolysis and associated AKI. In this study, we explored the role of umbelliferone against glycerol-induced AKI in rats. Kidney function was assessed by measuring serum creatinine, urea, electrolytes, and microproteinuria. Renal oxidative stress was quantified using thiobarbituric acid reactive substances, superoxide anion generation, and reduced glutathione assay. Renal histological changes were determined using periodic acid Schiff and hematoxylin-eosin staining, and immunohistology of apoptotic markers (Bax, Bcl-2) was done. Serum creatine kinase was quantified to assess glycerol-induced muscle damage. Umbelliferone attenuated glycerol-induced change in biochemical parameters, oxidative stress, histological alterations, and renal apoptosis. Pretreatment with bisphenol A diglycidyl ether, a peroxisome proliferator-activated receptor-γ (PPAR-γ) antagonist, attenuated umbelliferone-mediated protection. It is concluded that umbelliferone attenuates glycerol-induced AKI possibly through PPAR-γ agonism in rats.

中文翻译：

---

伞形酮通过大鼠过氧化物酶体增殖物激活受体-γ激动减轻甘油诱导的肌红蛋白尿急性肾损伤

横纹肌溶解症是一种由骨骼肌损伤引起的临床综合征，其因此将分解产物释放到循环中并导致人类急性肾损伤 (AKI)。肌内注射甘油可模拟横纹肌溶解症和相关的 AKI。在这项研究中，我们探讨了伞形酮对大鼠甘油诱导的 AKI 的作用。通过测量血清肌酐、尿素、电解质和微量蛋白尿来评估肾功能。使用硫代巴比妥酸反应物质、超氧阴离子产生和还原型谷胱甘肽测定对肾脏氧化应激进行量化。使用高碘酸希夫和苏木精-伊红染色确定肾脏组织学变化，并进行凋亡标志物（Bax，Bcl-2）的免疫组织学检查。量化血清肌酸激酶以评估甘油诱导的肌肉损伤。Umbelliferone 减弱甘油诱导的生化参数、氧化应激、组织学改变和肾细胞凋亡的变化。用双酚 A 二缩水甘油醚（一种过氧化物酶体增殖物激活受体-γ (PPAR-γ) 拮抗剂）预处理减弱了伞形酮介导的保护作用。得出的结论是，伞形酮可能通过 PPAR-γ 激动剂在大鼠中减弱甘油诱导的 AKI。