The tomato leaf miner, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), is a devastating pest worldwide affecting tomato production. Entomopathogenic nematodes (EPNs) are well known for their potential as biocontrol agents to control the emergence and development of this insect pest. Virulence and pathogenicity of five EPN strains isolated from different fields in Morocco were evaluated against the larval stage of T. absoluta. In laboratory assays, Steinernema feltiae strains (SF-MOR9 and SF-MOR10) showed significantly higher infectivity rates after 72 h compared to Heterorhabditis strains. In leaf bioassay, S. feltiae strains alongside H. bacteriophora (HB-MOR8) strain caused the highest larval mortality rate (80–100%) at 40–50 infective juveniles (IJs) cm⁻² which confirms the importance of the dose applied. On top of that, EPNs were able to locate and accurately kill the insect larvae inside and outside leaf conditions. Subsequently, the results showed that both S. feltiae strains were significantly effective in pot experiments for both applied concentrations (40 and 50 IJs cm⁻²). In addition, the efficiency of these nematodes was assessed under field conditions. Both S. feltiae accessions had optimal effects against T. absoluta larvae with more than 80% mortality rate at 50 IJs cm⁻². Heterorhabditis strain (HB-MOR8) significantly reduced larval occurrence with a more than 60% mortality rate when applied at the same dose. Therefore, the three tested indigenous EPN strains; SF-MOR9, SF-MOR10, and HB-MOR8 could be used as promising eco-friendly biological agents against T. absoluta in a broad agronomic range.

番茄潜叶虫Tuta absoluta (Meyrick)（鳞翅目：Gelechiidae）是世界范围内影响番茄生产的毁灭性害虫。昆虫病原线虫 (EPN) 因其作为生物防治剂的潜力而闻名，可控制这种害虫的出现和发展。针对T. absoluta的幼虫阶段评估了从摩洛哥不同田间分离的 5 种 EPN 菌株的毒力和致病性。在实验室试验中，斯氏线虫菌株（SF-MOR9和SF-MOR10）相比显示出72小时后显著更高的感染速率异小菌株。在叶生物测定中，毡合孢子菌与细菌菌群一起菌株(HB-MOR8) 菌株在 40-50 只感染性幼鱼 (IJs) cm ^-2处导致最高的幼虫死亡率（80-100%），这证实了所应用剂量的重要性。最重要的是，EPN 能够定位并准确杀死叶片内外的昆虫幼虫。随后，结果表明，S.氏线虫菌株显著有效盆栽试验两者施加的浓度（40和50 IJS厘米^-2）。此外，在田间条件下评估了这些线虫的效率。两种S. Feeliae种质均对T. absoluta幼虫具有最佳效果，在 50 IJs cm ^{-2 时}死亡率超过 80% 。异口癖菌株 (HB-MOR8) 以相同剂量应用时，显着降低了幼虫的发生率，死亡率超过 60%。因此，三个测试的本土 EPN 菌株；SF-MOR9、SF-MOR10 和 HB-MOR8 可作为有前景的生态友好型生物制剂，在广泛的农艺范围内对抗T. absoluta。