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JAZF1 heterozygous knockout mice show altered adipose development and metabolism
Cell and Bioscience ( IF 6.1 ) Pub Date : 2021-08-19 , DOI: 10.1186/s13578-021-00625-1
Jain Jeong 1 , Soyoung Jang 2 , Song Park 3, 4 , Wookbong Kwon 5 , Si-Yong Kim 2 , Soyoen Jang 2 , Jiwon Ko 2 , Si Jun Park 2 , Su-Geun Lim 2 , Duhak Yoon 6 , Junkoo Yi 7 , Sanggyu Lee 2 , Myoung Ok Kim 8 , Seong-Kyoon Choi 3, 5 , Zae Young Ryoo 2
Affiliation  

Juxtaposed with another zinc finger protein 1 (JAZF1) is associated with metabolic disorders, including type 2 diabetes mellitus (T2DM). Several studies showed that JAZF1 and body fat mass are closely related. We attempted to elucidate the JAZF1 functions on adipose development and related metabolism using in vitro and in vivo models. The JAZF1 expression was precisely regulated during adipocyte differentiation of 3T3-L1 preadipocyte and mouse embryonic fibroblasts (MEFs). Homozygous JAZF1 deletion (JAZF1-KO) resulted in impaired adipocyte differentiation in MEF. The JAZF1 role in adipocyte differentiation was demonstrated by the regulation of PPARγ—a key regulator of adipocyte differentiation. Heterozygous JAZF1 deletion (JAZF1-Het) mice fed a normal diet (ND) or a high-fat diet (HFD) had less adipose tissue mass and impaired glucose homeostasis than the control (JAZF1-Cont) mice. However, other metabolic organs, such as brown adipose tissue and liver, were negligible effect on JAZF1 deficiency. Our findings emphasized the JAZF1 role in adipocyte differentiation and related metabolism through the heterozygous knockout mice. This study provides new insights into the JAZF1 function in adipose development and metabolism, informing strategies for treating obesity and related metabolic disorders.

中文翻译:

JAZF1 杂合子敲除小鼠的脂肪发育和代谢发生改变

与另一种锌指蛋白 1 (JAZF1) 并列与代谢紊乱有关,包括 2 型糖尿病 (T2DM)。多项研究表明,JAZF1 与体脂肪量密切相关。我们尝试使用体外和体内模型阐明 JAZF1 在脂肪发育和相关代谢方面的功能。JAZF1 表达在 3T3-L1 前脂肪细胞和小鼠胚胎成纤维细胞 (MEF) 的脂肪细胞分化过程中受到精确调节。纯合 JAZF1 缺失(JAZF1-KO)导致 MEF 中脂肪细胞分化受损。JAZF1 在脂肪细胞分化中的作用通过 PPARγ(脂肪细胞分化的关键调节因子)的调节得到证实。与对照 (JAZF1-Cont) 小鼠相比,以正常饮食 (ND) 或高脂肪饮食 (HFD) 喂养的杂合 JAZF1 缺失 (JAZF1-Het) 小鼠具有更少的脂肪组织质量和受损的葡萄糖稳态。然而,其他代谢器官,如棕色脂肪组织和肝脏,对 JAZF1 缺乏的影响可以忽略不计。我们的研究结果强调了 JAZF1 通过杂合敲除小鼠在脂肪细胞分化和相关代谢中的作用。这项研究为 JAZF1 在脂肪发育和代谢中的功能提供了新的见解,为治疗肥胖和相关代谢紊乱的策略提供了信息。我们的研究结果强调了 JAZF1 通过杂合敲除小鼠在脂肪细胞分化和相关代谢中的作用。这项研究为 JAZF1 在脂肪发育和代谢中的功能提供了新的见解,为治疗肥胖和相关代谢紊乱的策略提供了信息。我们的研究结果强调了 JAZF1 通过杂合敲除小鼠在脂肪细胞分化和相关代谢中的作用。这项研究为 JAZF1 在脂肪发育和代谢中的功能提供了新的见解,为治疗肥胖和相关代谢紊乱的策略提供了信息。
更新日期:2021-08-19
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