Highly sensitive detection of paraquat with pillar[5]arenes as an aptamer in an α-hemolysin nanopore,Materials Chemistry Frontiers

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Highly sensitive detection of paraquat with pillar[5]arenes as an aptamer in an α-hemolysin nanopore
Materials Chemistry Frontiers ( IF 6.0 ) Pub Date : 2021-08-05 , DOI: 10.1039/d1qm00875g
Xiaojia Jiang ₁ , Mingsong Zang ₁ , Fei Li ₁ , Chunxi Hou ₁ , Quan Luo ₁ , Jiayun Xu ₂ , Junqiu Liu _{1,

2}

Affiliation

Recently, biological nanopore-based techniques have attracted more and more attention in the field of single-molecule detection because they allow real-time, sensitive, high-throughput analysis. Herein, we report an engineered biological nanopore sensor by introducing a macrocyclic host molecule carboxylatopillar[5]arene (CP[5]A) that acts as an aptamer into the lumen of a mutant (E111R/K147R)₇ αHL nanopore for detecting highly toxic paraquat (PQ) via host–guest interactions at the single-molecule level. By taking advantage of introducing positively charged arginine (Arg) into the lumen of the αHL nanopore, our engineered nanopore sensor exhibits higher stability. More importantly, this nanopore sensor shows high sensitivity, and the limit of detection (LOD) for PQ can reach a nanomolar level of 0.37 ppb. The CP[5]A-based nanopore sensing strategy developed in this work may bring inspiration for single-molecule detection and hold great potential in public health applications.

中文翻译：

以柱[5]芳烃为适体在α-溶血素纳米孔中高灵敏度检测百草枯

近年来，基于生物纳米孔的技术在单分子检测领域引起了越来越多的关注，因为它们可以实现实时、灵敏、高通量的分析。在此，我们通过将大环宿主分子羧基柱[5]芳烃（CP[5]A）作为适体引入突变体（E111R/K147R）₇ αHL纳米孔的管腔，报告了一种工程生物纳米孔传感器，用于检测高毒性百草枯 (PQ)通过单分子水平的主客体相互作用。通过将带正电荷的精氨酸 (Arg) 引入 αHL 纳米孔的管腔，我们设计的纳米孔传感器表现出更高的稳定性。更重要的是，这种纳米孔传感器显示出高灵敏度，PQ 的检测限 (LOD) 可以达到 0.37 ppb 的纳摩尔水平。这项工作中开发的基于CP[5]A的纳米孔传感策略可能为单分子检测带来灵感，并在公共卫生应用中具有巨大潜力。

更新日期：2021-08-19

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