Self-organizing gelatin–polycaprplactone materials with good fluid transmission can promote full-thickness skin regeneration,Materials Chemistry Frontiers

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Self-organizing gelatin–polycaprplactone materials with good fluid transmission can promote full-thickness skin regeneration
Materials Chemistry Frontiers ( IF 6.0 ) Pub Date : 2021-08-18 , DOI: 10.1039/d1qm00755f
Yifeng Nie _{1,

2} , Xinxiao Han ₁ , Zhuo Ao _{1,

2} , Shangwei Ning ₃ , Xiang Li _{1,

2} , Dong Han _{1,

2}

Affiliation

Healing, reconstruction and recovery after skin injury have long been a major field of research, and many biomaterials have been designed for skin healing treatment. Because the skin repair process includes a series of complex processes such as hemostasis, inflammatory reactions and tissue remodeling, the microenvironment for bionic cell growth is particularly important. Herein, we used the self-organization effect of natural polymer materials to simulate the extracellular matrix, and prepared a structure-stable gelatin–polycaprplactone (Gt–PCL) through high-voltage electrospinning technology and a multiphase method. The composite material was capable of phase separation and showed a superior water retention rate and good fluid transmission effects in vivo and in vitro, which was used in repairing tests after full-thickness skin excision in rats. Gt–PCL materials showed not only good biocompatibility but also appropriate mechanical properties to effectively improve the epidermal healing rate and prevent wound expansion. RNA-sequencing and differential expression analysis showed that the polycaprolactone biomimetic formed a stable multi-stage, micro–nano composite structure with various soluble amino acids or proteins through self-organization. This material was able to repair the skin by up-regulating tissue repair-related genes, initiating neural repair and hair regeneration pathways and down-regulating the immune response and stress response-related pathways, thus avoiding secondary injury and excessive wound repair. Moreover, Itgax, a key mRNA associated with wound healing, was identified and has been shown to affect the immune and barrier profiles of the skin. Together, these results demonstrate that Gt–PCL materials promote full-thickness skin regeneration, and may have good clinical effects and potential application value in the future.

中文翻译：

具有良好流体传输的自组织明胶-聚己内酯材料可促进全层皮肤再生

皮肤损伤后的愈合、重建和恢复长期以来一直是一个主要的研究领域，许多生物材料被设计用于皮肤愈合治疗。由于皮肤修复过程包括止血、炎症反应和组织重塑等一系列复杂过程，因此仿生细胞生长的微环境尤为重要。在此，我们利用天然高分子材料的自组织效应模拟细胞外基质，通过高压静电纺丝技术和多相法制备了结构稳定的明胶-聚己内酯（Gt-PCL）。该复合材料是能够相分离的，并呈现出优异的保水性速率和良好的流体传输效应在体内和体外，用于大鼠全层皮肤切除后的修复试验。Gt-PCL 材料不仅具有良好的生物相容性，而且具有适当的机械性能，可有效提高表皮愈合率并防止伤口扩张。RNA测序和差异表达分析表明，聚己内酯仿生物通过自组织与各种可溶性氨基酸或蛋白质形成稳定的多级微纳米复合结构。这种材料能够通过上调组织修复相关基因、启动神经修复和毛发再生途径以及下调免疫反应和应激反应相关途径来修复皮肤，从而避免继发性损伤和过度的伤口修复。此外，Itgax，一种与伤口愈合相关的关键 mRNA，已被鉴定并已被证明会影响皮肤的免疫和屏障特性。总之，这些结果表明Gt-PCL材料促进全层皮肤再生，未来可能具有良好的临床效果和潜在的应用价值。

更新日期：2021-08-19

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