SWCNTs/PEDOT:PSS-Modified Microelectrode Arrays for Dual-Mode Detection of Electrophysiological Signals and Dopamine Concentration in the Striatum under Isoflurane Anesthesia,ACS Sensors

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SWCNTs/PEDOT:PSS-Modified Microelectrode Arrays for Dual-Mode Detection of Electrophysiological Signals and Dopamine Concentration in the Striatum under Isoflurane Anesthesia
ACS Sensors ( IF 8.2 ) Pub Date : 2021-08-19 , DOI: 10.1021/acssensors.1c01241
Enhui He _{1,

2} , Shengwei Xu _{1,

2} , Yuchuan Dai _{1,

2} , Yiding Wang _{1,

2} , Guihua Xiao _{1,

2} , Jingyu Xie _{1,

2} , Shihong Xu _{1,

2} , Penghui Fan _{1,

2} , Fan Mo _{1,

2} , Mixia Wang _{1,

2} , Yilin Song _{1,

2} , Huabing Yin ₃ , Yinghui Li ₄ , Ying Wang ₅ , Xinxia Cai _{1,

2}

Affiliation

Accurate detection of the degree of isoflurane anesthesia during a surgery is important to avoid the risk of overdose isoflurane anesthesia timely. To address this challenge, a four-shank implantable microelectrode array (MEA) was fabricated for the synchronous real-time detection of dual-mode signals [electrophysiological signal and dopamine (DA) concentration] in rat striatum. The SWCNTs/PEDOT:PSS nanocomposites were modified onto the MEAs, which significantly improved the electrical and electrochemical performances of the MEAs. The electrical performance of the modified MEAs with a low impedance (16.20 ± 1.68 kΩ) and a small phase delay (−27.76 ± 0.82°) enabled the MEAs to detect spike firing with a high signal-to-noise ratio (> 3). The electrochemical performance of the modified MEAs with a low oxidation potential (160 mV), a low detection limit (10 nM), high sensitivity (217 pA/μM), and a wide linear range (10 nM–72 μM) met the specific requirements for DA detection in vivo. The anesthetic effect of isoflurane was mediated by inhibiting the spike firing of D2_SPNs (spiny projection neurons expressing the D2-type DA receptor) and the broadband oscillation rhythm of the local field potential (LFP). Therefore, the spike firing rate of D2_SPNs and the power of LFP could reflect the degree of isoflurane anesthesia together. During the isoflurane anesthesia-induced death procedure, we found that electrophysiological activities and DA release were strongly inhibited, and changes in the DA concentration provided more details regarding this procedure. The dual-mode recording MEA provided a detection method for the degree of isoflurane anesthesia and a prediction method for fatal overdose isoflurane anesthesia.

中文翻译：

SWCNTs/PEDOT：PSS 修饰的微电极阵列，用于双模式检测异氟醚麻醉下纹状体中的电生理信号和多巴胺浓度

在手术过程中准确检测异氟醚麻醉的程度对于及时避免异氟醚麻醉过量的风险很重要。为了应对这一挑战，制造了四柄可植入微电极阵列 (MEA)，用于同步实时检测大鼠纹状体中的双模式信号 [电生理信号和多巴胺 (DA) 浓度]。将 SWCNTs/PEDOT:PSS 纳米复合材料改性到 MEAs 上，显着提高了 MEAs 的电学和电化学性能。具有低阻抗 (16.20 ± 1.68 kΩ) 和小相位延迟 (-27.76 ± 0.82°) 的改良 MEA 的电气性能使 MEA 能够以高信噪比 (> 3) 检测尖峰发射。具有低氧化电位（160 mV）的改性 MEA 的电化学性能，低检测限 (10 nM)、高灵敏度 (217 pA/μM) 和宽线性范围 (10 nM–72 μM) 满足体内 DA 检测的特定要求。异氟醚的麻醉作用是通过抑制 D2_SPNs（表达 D2 型 DA 受体的棘突投射神经元）和局部场电位 (LFP) 的宽带振荡节律来介导的。因此，D2_SPNs 的尖峰放电率和 LFP 的功率可以共同反映异氟醚麻醉的程度。在异氟醚麻醉诱导的死亡过程中，我们发现电生理活动和 DA 释放受到强烈抑制，DA 浓度的变化提供了有关此过程的更多细节。

更新日期：2021-09-24

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