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Protein profiling in the habenula after chronic (–)-menthol exposure in mice
Journal of Neurochemistry ( IF 4.2 ) Pub Date : 2021-08-18 , DOI: 10.1111/jnc.15495 Matthew J Mulcahy 1 , Stephanie M Huard 1 , Joao A Paulo 2 , Jonathan H Wang 1 , Sheri McKinney 1 , Michael J Marks 1 , Brandon J Henderson 3 , Henry A Lester 1
Journal of Neurochemistry ( IF 4.2 ) Pub Date : 2021-08-18 , DOI: 10.1111/jnc.15495 Matthew J Mulcahy 1 , Stephanie M Huard 1 , Joao A Paulo 2 , Jonathan H Wang 1 , Sheri McKinney 1 , Michael J Marks 1 , Brandon J Henderson 3 , Henry A Lester 1
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The identification of proteins that are altered following nicotine/tobacco exposure can facilitate and positively impact the investigation of related diseases. In this report, we investigated the effects of chronic (–)-menthol exposure in 14 murine brain regions for changes in total β2 subunit protein levels and changes in epibatidine binding levels using immunoblotting and radioligand binding assays. We identified the habenula as a region of interest due to the region's marked decreases in β2 subunit and nAChR levels in response to chronic (–)-menthol alone. Thus, we further examined the habenula, a brain region associated with both the reward and withdrawal components of addiction, for additional protein level alterations using mass spectrometry. A total of 552 proteins with altered levels were identified after chronic (–)-menthol exposure. Enriched in the proteins with altered levels after (–)-menthol exposure were proteins associated with signaling, immune systems, RNA regulation, and protein transport. The continuation and expansion of the brain region-specific protein profiling in response to (–)-menthol will provide a better understanding of how this common flavorant in tobacco and e-liquid products may affect addiction and general health.
中文翻译：
小鼠慢性（-）-薄荷醇暴露后缰核中的蛋白质分析
识别在尼古丁/烟草暴露后发生改变的蛋白质可以促进并积极影响相关疾病的调查。在本报告中，我们使用免疫印迹和放射性配体结合测定研究了 14 个小鼠脑区慢性 (-)-薄荷醇暴露对总 β2 亚基蛋白水平变化和地巴替丁结合水平变化的影响。我们将缰核确定为感兴趣的区域，因为该区域单独响应慢性 (-)-薄荷醇会显着降低 β2 亚基和 nAChR 水平。因此，我们进一步检查了缰核，这是一个与成瘾的奖励和戒断成分相关的大脑区域，使用质谱法进行额外的蛋白质水平改变。在慢性 (-)-薄荷醇暴露后，共鉴定出 552 种水平改变的蛋白质。在 (-)-薄荷醇暴露后，蛋白质含量发生变化，富含与信号传导、免疫系统、RNA 调节和蛋白质转运相关的蛋白质。响应 (-)-薄荷醇的大脑区域特异性蛋白质谱的持续和扩展将提供更好的理解烟草和电子液体产品中这种常见的调味剂如何影响成瘾和整体健康。
更新日期：2021-09-22
中文翻译：
小鼠慢性（-）-薄荷醇暴露后缰核中的蛋白质分析
识别在尼古丁/烟草暴露后发生改变的蛋白质可以促进并积极影响相关疾病的调查。在本报告中，我们使用免疫印迹和放射性配体结合测定研究了 14 个小鼠脑区慢性 (-)-薄荷醇暴露对总 β2 亚基蛋白水平变化和地巴替丁结合水平变化的影响。我们将缰核确定为感兴趣的区域，因为该区域单独响应慢性 (-)-薄荷醇会显着降低 β2 亚基和 nAChR 水平。因此，我们进一步检查了缰核，这是一个与成瘾的奖励和戒断成分相关的大脑区域，使用质谱法进行额外的蛋白质水平改变。在慢性 (-)-薄荷醇暴露后，共鉴定出 552 种水平改变的蛋白质。在 (-)-薄荷醇暴露后，蛋白质含量发生变化，富含与信号传导、免疫系统、RNA 调节和蛋白质转运相关的蛋白质。响应 (-)-薄荷醇的大脑区域特异性蛋白质谱的持续和扩展将提供更好的理解烟草和电子液体产品中这种常见的调味剂如何影响成瘾和整体健康。
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