Background

Matrix metalloproteinase 10 (MMP-10) has a close relationship with carotid atherosclerosis (CAS) and cerebral infarction. The MMP-10 rs17435959 polymorphism causes a leucine to valine transition at codon 4 in exon 1 of the MMP-10 gene and may have functional effects.

Objectives

To investigate the relationship between the MMP-10 rs17435959 polymorphism and the formation and stability of CAS plaques.

Materials and Methods

The present case-control study contains 738 visitors who came to our health examination center for the first time. According to the carotid ultrasound examinations, visitors were classified into the vulnerable plaque group (41–86 years old, 141 male, 105 female), the stable plaque group (41–86 years old, 141 male, 105 female) and the no plaque group (41–85 years old, 141 male, 105 female). All visitors in the three groups were sex- and- age-matched, and cardiovascular and cerebrovascular diseases were absent. The polymorphism was genotyped by real-time polymerase chain reaction- restriction.

Results

Compared to the GG genotype, the frequency of the CC and CG genotypes was significantly more common in the vulnerable plaque group than in the no plaque group (18.7% vs. 7.7%, unadjusted P = 0.002). Moreover, compared to the G allele, the frequency of the C allele was significantly more common in the vulnerable plaque group than in the no plaque group (10.4% vs. 3.9%, unadjusted P = 0.000) and in the vulnerable plaque group than in the stable plaque group (10.4% vs. 5.1%, unadjusted P = 0.008). Binary logistic regression showed that the CC and CG genotype was independent risk factor for the formation (P = 0.019, OR = 1.961, 95% CI [1.117, 3.444]) and vulnerability (P = 0.035, OR = 1.842, 95% CI [1.045, 3.247]) of CAS plaques. Moreover, individuals who have the C allele showed a higher level of fibrinogen, which was an independent risk factor for the formation of CAS plaques (P = 0.000, OR = 2.425, 95% CI [1.475, 3.985]).

Conclusions

The rs17435959 polymorphism was associated with the formation and vulnerability of CAS plaques. Individuals who had variant-type MMP-10 showed higher levels of fibrinogen, which promoted the formation of CAS plaques.

中文翻译：

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MMP-10 rs17435959 多态性与颈动脉粥样硬化斑块的形成和稳定性相关：病例对照研究

背景

基质金属蛋白酶10（MMP-10）与颈动脉粥样硬化（CAS）和脑梗塞有密切关系。所述MMP-10 rs17435959多态性导致在密码子4的外显子中的MMP-10基因的1亮氨酸至缬氨酸过渡，并且可以具有的作用效果。

目标

探讨MMP-10 rs17435959多态性与CAS斑块形成及稳定性的关系。

材料和方法

本病例对照研究包含 738 名首次来到我们健康检查中心的访客。根据颈动脉超声检查，将参观者分为易损斑块组（41-86岁，男性141人，女性105人），稳定斑块组（41-86岁，男性141人，女性105人）和无斑块组（41-85岁，男141人，女105人）。三组来访者均性别、年龄相匹配，均未出现心脑血管疾病。多态性通过实时聚合酶链反应限制进行基因分型。

结果

与 GG 基因型相比，易损斑块组中 CC 和 CG 基因型的频率明显高于无斑块组（18.7% vs. 7.7%，未调整P  = 0.002）。此外，与 G 等位基因相比，易损斑块组中 C 等位基因的频率明显高于无斑块组（10.4% 对 3.9%，未调整P  = 0.000）和易损斑块组稳定斑块组（10.4% vs. 5.1%，未调整P  = 0.008）。二元逻辑回归显示CC和CG基因型是形成（P  = 0.019，OR  =  1.961，95 ％  CI [1.117，3.444]）和脆弱性（P = 0.035, OR  = 1.842, 95%  CI  [1.045, 3.247]) CAS 斑块。此外，具有 C 等位基因的个体显示出更高水平的纤维蛋白原，这是形成 CAS 斑块的独立危险因素（P  = 0.000，OR  = 2.425，95 % CI [1.475, 3.985]）。

结论

rs17435959 多态性与 CAS 斑块的形成和易损性有关。具有变异型 MMP-10 的个体显示出更高水平的纤维蛋白原，这促进了 CAS 斑块的形成。