Senescent cells in 3D culture show suppressed senescence signatures,Biomaterials Science

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Senescent cells in 3D culture show suppressed senescence signatures
Biomaterials Science ( IF 5.8 ) Pub Date : 2021-08-03 , DOI: 10.1039/d1bm00536g
Parul Yadav ₁ , Kaushik Chatterjee _{1,

2} , Deepak Kumar Saini _{1,

3}

Affiliation

Cellular senescence, an irreversible proliferation arrested but viable cellular state, has been implicated in the progression of several age-associated pathologies. A vast amount of information about senescence has been acquired in cultured cells; however, senescence in living organisms (in vivo) remains poorly understood, mainly because of technical limitations. Furthermore, it is now widely recognized that three-dimensional (3D) culture systems are a better mimic of the in vivo physiology. Herein, senescence was induced in HeLa cells by irradiation. Non-senescent or senescent cells were cultured in soft 3D polymer scaffolds and compared with cells in conventional two-dimensional (2D) culture. This work shows that the morphology of the senescent cells markedly varies between substrates/culture platforms, driving the differences in the cytoskeletal organization, cellular division, and nanomechanical properties. One characteristic feature of senescent cells on 2D culture systems is the enlarged and flattened morphology; however, such drastic changes are not seen in vivo. This is an artificial effect of the substrate, which renders such non-physiological morphology to senescent cells. In the 3D scaffolds, this artifact is reduced. Hence, it serves as a better mimic of tissues, leading to reduced expression of senescence-associated genes, implying that the 3D scaffolds suppress the senescence in cells.

中文翻译：

3D 培养中的衰老细胞显示出抑制的衰老特征

细胞衰老是一种不可逆的增殖停滞但仍可存活的细胞状态，与几种与年龄相关的病理的进展有关。在培养细胞中获得了大量关于衰老的信息；然而，生物体（体内）的衰老仍然知之甚少，主要是由于技术限制。此外，现在人们普遍认识到，三维 (3D) 培养系统可以更好地模拟体内生理。在本文中，通过辐射在 HeLa 细胞中诱导衰老。非衰老或衰老细胞在软 3D 聚合物支架中培养，并与传统二维 (2D) 培养中的细胞进行比较。这项工作表明，衰老细胞的形态在基质/培养平台之间存在显着差异，从而导致细胞骨架组织、细胞分裂和纳米力学特性的差异。二维培养系统上衰老细胞的特征之一是扩大和扁平的形态；然而，这种剧烈的变化在体内是看不到的. 这是底物的人工作用，它使衰老细胞呈现这种非生理形态。在 3D 支架中，这种伪影减少了。因此，它可以更好地模拟组织，导致衰老相关基因的表达减少，这意味着 3D 支架抑制细胞衰老。

更新日期：2021-08-19

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