Phytochemical study of Rourea oligophlebia stems led to the isolation of a new 2-pyrrolidone alkaloid (R,S)-N-(5-hydroxyl-pyrrolidin-2-one-1-yl)acetamide (1), together with 14 known compounds including friedelin (2), friedanol (3), taraxerol (4), vanillin (5), coniferyl aldehyde (6), apigenin (7), 7α-hydroxy-3β-sitosterol (8), coniferyl alcohol (9), scopoletin (10), emodin (11), protocatechuic acid (12), catechin (13), procyanidin A1 (14), and (E)-2,3,5,4’-tetrahydroxystilbene-2-β-D-glucoside (15). Several isolated compounds were evaluated for cytotoxicity and antimicrobial activity. Compound 11 exhibited good antimicrobial activity on Gram (+) strains and moderate cytotoxicity against KB, Hep-G2, and LU cancer cell lines. Compounds 6 and 8–10 showed selective activity on HepG-2 and MCF-7 over KB and LU cancer cell lines, while compound 7 exhibited similar effects on KB, HepG-2, and MCF-7 cell lines with IC₅₀ values of 36.46 ± 0.81, 32.00 ± 0.58, and 32.03 ± 0.61 µg/mL, respectively.

中文翻译：

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来自 Rourea oligophlebia 茎的一种新的吡咯烷酮生物碱和其他成分

Rourea oligophlebia茎的植物化学研究导致分离出一种新的 2-吡咯烷酮生物碱 ( R , S )- N -(5-hydroxyl-pyrrolidin-2-one-1-yl) 乙酰胺 ( 1 )，以及 14 种已知化合物包括木栓（2），friedanol（3），蒲公英赛醇（4），香草醛（5），松柏醛（6），芹菜素（7），7 α -羟基-3 β谷甾醇（8），松柏醇（9） , 东莨菪碱 ( 10 ), 大黄素 ( 11 ), 原儿茶酸 (12 )、儿茶素( 13 )、原花青素A1( 14 )和( E )-2,3,5,4'-四羟基二苯乙烯-2- β -D-葡萄糖苷( 15 )。评估了几种分离的化合物的细胞毒性和抗菌活性。化合物11对革兰氏 (+) 菌株表现出良好的抗菌活性，对 KB、Hep-G2 和 LU 癌细胞系具有中等的细胞毒性。化合物6和8 – 10对 HepG-2 和 MCF-7 显示出对 KB 和 LU 癌细胞系的选择性活性，而化合物7对 KB、HepG-2 和 MCF-7 细胞系表现出相似的作用，IC ₅₀值分别为 36.46 ± 0.81、32.00 ± 0.58 和 32.03 ± 0.61  µ g/mL。