The antibacterial property of silver nanoparticles (SNP) for combating antibiotic resistant bacteria has become increasingly important as a result of the emergence of multiple drug resistant pathogens. Due to a broader antibacterial range and decreased production doses, a combination of antibacterial agents and SNPs may prove to be more effective. Bacteriocin capped silver nanoparticles was characterized by UV–visible (UV–vis) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), and transmission electron microscope (TEM) and biological applications of antibiofilm assay and bactericidal activity of SEM analysis. The biological assay of BCSNPs on these cells is evaluated by cell proliferation, Wound scratch assay, and apoptotic assay. The SEM and TEM images are formation of the BCSNPs with spherical, distinct, and smooth surfaces and small sizes of 16 nm–22 nm in diameter, respectively, with small peptides present on this surface. The biofilm biomass reduced to 80–90% was observed in methicillin-resistant Staphylococcus aureus (MRSA) treated with BCSNPs due to its improved binding with bacterial surface. The invitro results demonstrated the concentration dependent toxicity for the cells tested and the concentration was determined as 40–50 µg/mL in 3T3 cells. Finally, the results showed that BCSNPs possess low toxicity to normal cells. The synthesized BCSNPs biological innovation and economically strength in this formulation and its small size of nanoparticles reacted fast and low toxicity to normal mice fibroblast 3T3 cell. In future, application can be used in medicine.

由于多种耐药病原体的出现，银纳米粒子 (SNP) 对抗抗生素耐药细菌的抗菌性能变得越来越重要。由于更广泛的抗菌范围和更低的生产剂量，抗菌剂和 SNP 的组合可能被证明更有效。通过紫外-可见（UV-vis）光谱、X射线衍射（XRD）、扫描电子显微镜（SEM）、能量色散X射线光谱（EDS）和透射电子显微镜（TEM）对细菌素封端的银纳米粒子进行了表征。抗生物膜测定的生物学应用和 SEM 分析的杀菌活性。BCSNPs 对这些细胞的生物学检测通过细胞增殖、伤口划痕检测和细胞凋亡检测进行评估。SEM 和 TEM 图像分别形成了具有球形、独特和光滑表面和直径为 16 nm-22 nm 的小尺寸的 BCSNP，该表面上存在小肽。在耐甲氧西林中观察到生物膜生物量减少到 80-90%用 BCSNPs 处理金黄色葡萄球菌(MRSA)，因为它改善了与细菌表面的结合。体外结果证明了所测试细胞的浓度依赖性毒性，并且在 3T3 细胞中的浓度确定为 40–50 µg/mL。最后，结果表明BCSNPs对正常细胞具有低毒性。该配方中合成的BCSNPs生物创新和经济实力及其小尺寸的纳米颗粒对正常小鼠成纤维细胞3T3细胞反应快速且毒性低。将来，应用可用于医学。