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Suitability of PEG capped carboxylic acid terminated fluorescent ZnS nanoparticles for NDV peptide binding
Applied Nanoscience Pub Date : 2021-08-17 , DOI: 10.1007/s13204-021-02013-0
N. Deepa 1 , A. Vimala Juliet 1 , K. Aanantharaj 2 , Jositta Sherine 2 , R. Sujith 3
Affiliation  

Newcastle disease, an economically important endemic OIE (World Organisation for Animal Health) listed viral disease remains a major impediment to the vibrant status of the poultry industry. The outbreak of the disease leads to huge economic loss posing a major threat to the Country’s economy. To detect the disease outbreak, an effective tool is required. The present study is a first step in forming a peptide nanoparticle conjugate as an attempt to develop a biosensor for NDV outbreak detection. ZnS due to its emission properties and photo stability, it was used as fluorescent labels in immunoassays. ZnS is widely used as the shell material in most luminescent core–shell nanoparticles. In this study, ZnS was used as the core material and the ZnS–PEG modified with the carboxylic acid group functionalized nanoparticles were prepared by a simple wet chemical method. To the ZnS–PEG–COOH nanoparticle, Newcastle disease virus peptide was attached using EDC/NHS coupling and analyzed. Structural properties of the ZnS, ZnS–PEG and ZnS–PEG–COOH nanoparticles were analyzed using X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and dynamic light scattering (DLS). FTIR peak at 2364 cm−1 indicates the formation of S–H bond which confirms the attachment of PEG to ZnS surface by S–H bond formation. The XRD, FTIR, DLS and optical study such as UV–Vis results support the particle size reduction upon capping ZnS with PEG and dicarboxylic-terminated PEG. The optical properties were studied using Photoluminescence (PL) and UV–visible Spectra. PL result shows upon capping ZnS with PEG-COOH, the fluorescence intensity increased by ≈ 5 times more than that of the bare ZnS nanoparticles. ZnS–PEG–COOH found to have superior photo luminescent property. NDV peptides were tagged by means of EDC/NHS activation to ZnS–PEG–COOH. The peptide conjugation was confirmed by UV–Vis spectral analysis and the peak was found to be shifted by 4 nm which confirms the attachment of NDV peptide on the surface of ZnS–PEG–COOH nanoparticle. Hence ZnS–PEG–COOH nanoparticle is found to be suitable option for attaching biomolecule which can be further extended for bio sensing application of NDV detection.



中文翻译:

PEG 封端的羧酸终止荧光 ZnS 纳米粒子对 NDV 肽结合的适用性

新城疫是一种在经济上很重要的地方性流行病 OIE(世界动物卫生组织)列出的病毒性疾病,它仍然是家禽业蓬勃发展的主要障碍。该病的爆发导致巨大的经济损失,对国家经济构成重大威胁。为了检测疾病的爆发,需要一种有效的工具。本研究是形成肽纳米颗粒缀合物的第一步,以尝试开发用于 NDV 爆发检测的生物传感器。ZnS 由于其发射特性和光稳定性,它被用作免疫测定中的荧光标记。ZnS 被广泛用作大多数发光核壳纳米粒子的壳材料。在这项研究中,以ZnS为核心材料,通过简单的湿化学方法制备了羧酸基官能化纳米颗粒修饰的ZnS-PEG。使用 EDC/NHS 偶联将新城疫病毒肽连接到 ZnS-PEG-COOH 纳米颗粒并进行分析。使用 X 射线衍射 (XRD)、傅里叶变换红外光谱 (FTIR)、扫描电子显微镜 (SEM) 和动态光散射 (DLS) 分析了 ZnS、ZnS-PEG 和 ZnS-PEG-COOH 纳米颗粒的结构特性。2364 cm 处的 FTIR 峰 扫描电子显微镜 (SEM) 和动态光散射 (DLS)。2364 cm 处的 FTIR 峰 扫描电子显微镜 (SEM) 和动态光散射 (DLS)。2364 cm 处的 FTIR 峰−1表明 S-H 键的形成,这证实了 PEG 通过 S-H 键形成连接到 ZnS 表面。XRD、FTIR、DLS 和光学研究(如 UV-Vis 结果)支持用 PEG 和二羧酸封端的 PEG 封端 ZnS 后粒径减小。使用光致发光 (PL) 和紫外-可见光谱研究光学特性。PL 结果显示,用 PEG-COOH 覆盖 ZnS 后,荧光强度比裸 ZnS 纳米粒子的荧光强度增加约 5 倍。发现 ZnS-PEG-COOH 具有优异的光致发光性能。NDV 肽通过 EDC/NHS 激活到 ZnS-PEG-COOH 进行标记。UV-Vis 光谱分析证实了肽缀合,发现峰移动了 4 nm,这证实了 NDV 肽附着在 ZnS-PEG-COOH 纳米颗粒表面。

更新日期:2021-08-19
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