The present study was conducted to evaluate Averrhoa bilimbi fruits flavonoids extraction and quantification analysis through RP-HPLC and its comparison study on total flavonoids concentration versus antimicrobial activity analysis based on minimum inhibitory concentrations. Optimization of extraction was carried out using three different methods; among all methods the ultrasonic conventional assistant extraction (UCAE) showed an excellent recovery of flavonoids with solid phase elution. UCAE was performed with ethanol using different solvents ratio, the total flavonoid content was quantified through spectrophotometrically (850 ± 25 mg/kg) and flavonoids (myricetin and luteolin) were quantified by RP-HPLC. Optimized yield of myricetin and luteolin were presented in A. bilimbi fruits 336 ± 15 and 231 ± 18 mg/kg, respectively. Antimicrobial activity examined against E. coli, S. aureus and B. subtilis species using spectroscopically. The extracted sample with known quantity of total flavonoids content (10–200 µg/mL) used against E. coli, S. aureus and B. subtilis, MIC results shows 55.6 ± 6, 31 ± 3 and 28 ± 2 µg/mL respectively. Higher flavonoid content plays major role on antioxidant activities, which were evaluated and compared with commercial antioxidant butylated hydroxytoluene (BHT) employing superoxide anion scavenging activity and total reducing power IC₅₀ value results shows 100 and 175 µg/mL, respectively. The maximum yield of flavonoid content results shows method suitability of flavonoids extraction and quantification.

本研究的目的是通过 RP-HPLC 评估Averrhoa bilimbi果实类黄酮的提取和定量分析，以及基于最低抑菌浓度的总黄酮浓度与抗菌活性分析的比较研究。使用三种不同的方法进行提取优化；在所有方法中，超声波常规辅助提取 (UCAE) 显示出黄酮类化合物在固相洗脱方面的出色回收率。使用不同溶剂比例的乙醇进行 UCAE，通过分光光度法 (850 ± 25 mg/kg) 对总类黄酮含量进行定量，并通过 RP-HPLC 对类黄酮（杨梅素和木犀草素）进行定量。杨梅素和木犀草素的优化产量在A. bilimbi中呈现水果分别为 336 ± 15 和 231 ± 18 mg/kg。使用光谱法检查针对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌的抗菌活性。已知总黄酮含量（10-200 µg/mL）的提取样品用于对抗大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌，MIC 结果分别为 55.6 ± 6、31 ± 3 和 28 ± 2 µg/mL . _{较高的类黄酮含量对抗氧化活性起着重要作用，通过超氧阴离子清除活性和总还原力 IC 50}评估并与商业抗氧化剂丁基化羟基甲苯 (BHT) 进行比较值结果分别显示 100 和 175 µg/mL。黄酮含量的最大得率结果表明黄酮提取和定量方法的适用性。