Can diazirine-modified lipids be used to locate the position of UV-induced cross-links between this lipid and a membrane peptide by using MS? Although DiazPC fulfils all prerequisites for a photo-activatable membrane lipid to study protein/lipid interactions and the cross-links can be exactly assigned, we observed a backfolding of the lipid's alkyl chain. This is a general phenomenon for any type of membrane lipids with a hydrophilic group in the alkyl chain.

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用于研究肽/脂质相互作用的二氮嗪修饰的膜脂质——机遇和挑战

可以使用二氮嗪修饰的脂质通过 MS 定位该脂质和膜肽之间紫外线诱导的交联的位置吗？尽管 DiazPC 满足光活化膜脂研究蛋白质/脂质相互作用的所有先决条件，并且可以准确分配交联，但我们观察到脂质烷基链的回折叠。这是烷基链中具有亲水基团的任何类型膜脂的普遍现象。