Journal of Cluster Science ( IF 2.7 ) Pub Date : 2021-08-18 , DOI: 10.1007/s10876-021-02154-w Huy L. Nguyen 1 , Van-Anh Nguyen 1 , Duy T. Bui 1 , Ha Hong Cao 1 , Nam Thi Pham 2 , Dzung T. Nguyen 2 , Tran Dai Lam 2, 3 , Quan P. Do 4
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This work presents a design of a simple and sensitive sensing interface consisting of a graphene/poly(1,5-diaminonaphthalene) nanocomposite, denoted as Gr/P(1,5DAN), as an immobilization matrix and graphene decorated with silver nanoparticles (Gr-AgNPs) as an electrochemical label for bisphenol A (BPA). The nanocomposite was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and electrochemical techniques (CV and EIS). Immunocomplex formation between the anti-BPA antibody (αBPA) and BPA antigen would induce the square wave voltammetric (SWV) peak current variation of Gr-AgNPs labels, and afterward, the concentration of BPA could be indirectly assayed and qualitatively determined. The results indicated a linear relationship between (SWV) response to the BPA concentration in a range of 1.0–8.0 nM, with a limit of detection (LOD) of 0.6 nM. It is a promising attempt for PBA analysis with a low-cost and easy-to-fabricate sensor.
Graphic Abstract
中文翻译：
用银纳米颗粒装饰的石墨烯作为非酶促双酚 A 免疫传感器中的电催化标记
这项工作提出了一种简单而灵敏的传感界面的设计，该界面由石墨烯/聚（1,5-二氨基萘）纳米复合材料（表示为 Gr/P(1,5DAN)）组成，作为固定基质和用银纳米粒子装饰的石墨烯（Gr -AgNPs) 作为双酚 A (BPA) 的电化学标记。通过扫描电子显微镜 (SEM)、透射电子显微镜 (TEM) 和电化学技术 (CV 和 EIS) 表征纳米复合材料。抗 BPA 抗体 (αBPA) 和 BPA 抗原之间的免疫复合物形成会诱导 Gr-AgNPs 标记的方波伏安 (SWV) 峰值电流变化, 然后可以间接测定和定性测定 BPA 的浓度。结果表明 (SWV) 对 BPA 浓度的响应在 1.0–8.0 nM 范围内呈线性关系，检测限 (LOD) 为 0.6 nM。这是使用低成本且易于制造的传感器进行 PBA 分析的有希望的尝试。
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