Organ-on-a-chip technology, a promising three-dimensional (3D) dynamic culture method, ensures accurate and efficient cell culture and has great potential for replacing animal models in preclinical testing. The circulatory system, the most abundant organ in the human body, plays a crucial role in oxygen exchange and mass transfer, which is the determining factor for the survival of tissues and organs. Thus, it is essential to integrate the circulatory system into an organ-on-a-chip to recreate tissue and organ microenvironments and physiological functions. This review discusses the synergy between the vasculature and the emerging organ-on-a-chip technology, which offers even better possibilities of duplicating physiology and disease characteristics. In addition, we review the different steps of a vascularized organ-on-a-chip fabrication process, including structure fabrication and tissue construction using different biofabrication strategies. Finally, we outline the applicability of this technology in the fascinating and fast-developing field of organ and tumor culture.

器官芯片技术是一种很有前途的三维（3D）动态培养方法，可确保准确高效的细胞培养，在临床前试验中具有替代动物模型的巨大潜力。循环系统是人体内最丰富的器官，在氧气交换和质量传递中起着至关重要的作用，是组织器官生存的决定性因素。因此，必须将循环系统集成到器官芯片中，以重建组织和器官微环境和生理功能。这篇综述讨论了脉管系统和新兴的器官芯片技术之间的协同作用，这为复制生理学和疾病特征提供了更好的可能性。此外，我们回顾了带血管的芯片上器官制造过程的不同步骤，包括使用不同生物制造策略的结构制造和组织构建。最后，我们概述了这项技术在器官和肿瘤培养这个引人入胜且快速发展的领域的适用性。